Detailed information of TA system TA035393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 342933..343548
• Replicon: chromosome
• Accession: NZ_AP022347
• Organism: Fluviibacter phosphoraccumulans strain ICHIJ1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ICHIJ1_RS01795
• Protein ID: WP_162049024.1
• Coordinates: 342933..343238 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ICHIJ1_RS01800
• Protein ID: WP_162049025.1
• Coordinates: 343231..343548 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICHIJ1_RS01780	338719..339756	+	1038	WP_162049022.1	UDP-glucose 4-epimerase GalE	-
ICHIJ1_RS01785	340111..341670	+	1560	WP_202930726.1	Fic family protein	-
ICHIJ1_RS01790	341774..342712	-	939	WP_162049023.1	tyrosine-type recombinase/integrase	-
ICHIJ1_RS01795	342933..343238	+	306	WP_162049024.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICHIJ1_RS01800	343231..343548	+	318	WP_162049025.1	putative addiction module antidote protein	Antitoxin
ICHIJ1_RS01805	343722..344258	+	537	WP_162049026.1	hypothetical protein	-
ICHIJ1_RS01810	344328..344513	+	186	WP_162049027.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
ICHIJ1_RS12165	344692..344949	+	258	WP_202930725.1	hypothetical protein	-
ICHIJ1_RS12170	345010..345342	+	333	WP_202930724.1	AAA family ATPase	-
ICHIJ1_RS01820	345446..345709	+	264	WP_162049028.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
ICHIJ1_RS01825	345696..345977	+	282	WP_162049029.1	type II toxin-antitoxin system YafQ family toxin	-
ICHIJ1_RS01830	346288..347133	+	846	WP_162049030.1	NYN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	332167..347133	14966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (8-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (50-91)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11468.41 Da
• Isoelectric Point: 9.9485

>T35393 WP_162049024.1 NZ_AP022347:342933-343238 [Fluviibacter phosphoraccumulans]
MYTVIPIPAFSDWLRAIKDRPTQVRLMKRLRKAQLGNLGDVKQVGSGVWEMREFFGPGWRMYYIQRGDLLIVMLGGGDKS
TQSADIAKAISLANLMEIEHD

Nucleotide

• Length: 306 bp

>T35393 NZ_AP022347:342933-343238 [Fluviibacter phosphoraccumulans]
ATGTATACCGTTATACCAATCCCGGCCTTTTCTGACTGGCTCCGAGCCATTAAAGATCGCCCGACACAGGTTCGTCTGAT
GAAGCGCCTTCGTAAAGCGCAATTAGGCAATCTTGGGGATGTTAAACAGGTGGGCAGTGGTGTTTGGGAGATGCGGGAGT
TTTTCGGCCCCGGCTGGCGTATGTATTACATTCAGCGTGGGGATCTGTTGATCGTGATGCTGGGTGGTGGTGATAAATCC
ACGCAGTCAGCGGATATCGCCAAAGCAATTTCTCTAGCGAACCTAATGGAGATTGAACATGACTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11476.19 Da
• Isoelectric Point: 5.1568

>AT35393 WP_162049025.1 NZ_AP022347:343231-343548 [Fluviibacter phosphoraccumulans]
MTKKIKLSDCRPFDIAEYLDDEAMITEYMNSVLEENDPAMLASALGDIARARGMTQLAKETGLSRESLYKGLSGDRIPST
ETLLKVIHALGFKLSVSKADTVAHA

Nucleotide

• Length: 318 bp

>AT35393 NZ_AP022347:343231-343548 [Fluviibacter phosphoraccumulans]
ATGACTAAGAAGATTAAGCTTTCCGACTGCCGTCCATTTGATATTGCAGAATATCTGGACGATGAAGCCATGATTACCGA
GTACATGAACTCGGTCCTGGAAGAGAACGACCCAGCGATGCTGGCTTCAGCATTAGGCGATATCGCCCGTGCTCGTGGCA
TGACCCAATTGGCGAAGGAAACAGGCCTCAGCAGGGAAAGCCTATATAAGGGTTTATCGGGTGACCGTATCCCAAGCACT
GAAACGCTGCTGAAGGTAATTCACGCACTGGGATTCAAGTTGTCGGTATCTAAAGCGGATACAGTCGCACACGCTTGA