Detailed information of TA system TA035386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1711938..1712539
• Replicon: chromosome
• Accession: NZ_AP022345
• Organism: Fluviibacter phosphoraccumulans strain ICHIAU1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: ICHIAU1_RS08580
• Protein ID: WP_162049885.1
• Coordinates: 1711938..1712240 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ICHIAU1_RS08585
• Protein ID: WP_162049884.1
• Coordinates: 1712240..1712539 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICHIAU1_RS08550	1708152..1709615	-	1464	WP_162049887.1	transglycosylase SLT domain-containing protein	-
ICHIAU1_RS08570	1710199..1711605	-	1407	WP_162049886.1	glutamate--tRNA ligase	-
ICHIAU1_RS08580	1711938..1712240	+	303	WP_162049885.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICHIAU1_RS08585	1712240..1712539	+	300	WP_162049884.1	putative addiction module antidote protein	Antitoxin
ICHIAU1_RS08590	1712559..1715696	+	3138	WP_162071331.1	N-6 DNA methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-95)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11535.29 Da
• Isoelectric Point: 8.9004

>T35386 WP_162049885.1 NZ_AP022345:1711938-1712240 [Fluviibacter phosphoraccumulans]
MKIIYTTKIFDSWYQKLKDREAARFIQVRIDRAEDGNFGDCKPIGEGVSEMRLHVGPGYRLYFMQQGLEIVILLAGGNKS
SQSRDIEIALQIAREIRENK

Nucleotide

• Length: 303 bp

>T35386 NZ_AP022345:1711938-1712240 [Fluviibacter phosphoraccumulans]
ATGAAAATCATTTATACAACGAAGATCTTTGATTCTTGGTATCAAAAGCTCAAAGATCGGGAAGCGGCTCGGTTCATTCA
GGTGCGAATTGATCGCGCAGAAGATGGCAACTTTGGTGATTGCAAGCCCATTGGTGAAGGCGTATCTGAAATGCGTTTGC
ATGTAGGGCCAGGCTACCGTCTGTATTTTATGCAACAAGGTTTAGAGATTGTGATTTTGCTGGCAGGCGGAAACAAATCG
TCACAGAGCCGAGATATAGAGATTGCTTTGCAAATTGCAAGAGAGATTAGGGAGAACAAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10681.28 Da
• Isoelectric Point: 6.7170

>AT35386 WP_162049884.1 NZ_AP022345:1712240-1712539 [Fluviibacter phosphoraccumulans]
MKTIQLRRWDSAEHLKTPEDMALYLEACLQEAGDDAAFVAKALGNIARAKGMSQLANETGMGRESLYKALSGEGNPSFAT
ILKVTRALGIELHALPAHV

Nucleotide

• Length: 300 bp

>AT35386 NZ_AP022345:1712240-1712539 [Fluviibacter phosphoraccumulans]
ATGAAAACCATTCAGTTACGTCGCTGGGATAGTGCCGAACACCTTAAAACGCCGGAGGATATGGCGTTATATCTCGAGGC
TTGTCTCCAAGAAGCGGGAGACGACGCAGCATTTGTAGCCAAAGCACTAGGTAATATCGCACGCGCCAAAGGCATGTCGC
AGCTTGCTAATGAAACAGGTATGGGGCGTGAAAGTCTTTATAAAGCGCTATCGGGTGAAGGTAACCCAAGTTTTGCCACG
ATCCTTAAGGTTACCCGTGCGCTCGGCATTGAGCTGCATGCTCTGCCAGCTCACGTCTAA