Detailed information of TA system TA035385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1586528..1587129
• Replicon: chromosome
• Accession: NZ_AP022345
• Organism: Fluviibacter phosphoraccumulans strain ICHIAU1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: ICHIAU1_RS07945
• Protein ID: WP_162050014.1
• Coordinates: 1586528..1586833 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ICHIAU1_RS07950
• Protein ID: WP_162050013.1
• Coordinates: 1586830..1587129 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICHIAU1_RS07905	1581992..1582312	+	321	WP_162050019.1	hypothetical protein	-
ICHIAU1_RS07915	1582505..1582795	+	291	WP_162050018.1	nucleotidyltransferase family protein	-
ICHIAU1_RS07920	1582792..1583136	+	345	WP_202930671.1	DUF86 domain-containing protein	-
ICHIAU1_RS07925	1583340..1584487	+	1148	WP_162050017.1	IS3 family transposase	-
ICHIAU1_RS07930	1584516..1585181	-	666	WP_162050016.1	hypothetical protein	-
ICHIAU1_RS07935	1585527..1585802	+	276	WP_162050015.1	hypothetical protein	-
ICHIAU1_RS07940	1586037..1586258	+	222	WP_202930672.1	DUF4224 domain-containing protein	-
ICHIAU1_RS07945	1586528..1586833	+	306	WP_162050014.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICHIAU1_RS07950	1586830..1587129	+	300	WP_162050013.1	putative addiction module antidote protein	Antitoxin
ICHIAU1_RS07955	1587153..1587464	-	312	WP_162050012.1	hypothetical protein	-
ICHIAU1_RS07960	1587469..1588686	-	1218	WP_162050011.1	MFS transporter	-
ICHIAU1_RS07965	1588841..1589578	+	738	WP_162050010.1	3-ketoacyl-ACP reductase	-
ICHIAU1_RS07970	1589660..1590082	-	423	WP_162050942.1	DUF1841 family protein	-
ICHIAU1_RS07975	1590099..1590758	-	660	WP_162050009.1	endonuclease III	-
ICHIAU1_RS07980	1590771..1591460	-	690	WP_174237203.1	electron transport complex subunit E	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-92)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11439.08 Da
• Isoelectric Point: 9.9574

>T35385 WP_162050014.1 NZ_AP022345:1586528-1586833 [Fluviibacter phosphoraccumulans]
MKLIYTTEIFDAWFGRLKDKEVARRIQIRIDRAEDGHFGDCKSVGGGVSEMRIHHGPGYRVYFMQRGFEVVILLAGGDKS
SQSRDIQTAIHIAQQIKGSRS

Nucleotide

• Length: 306 bp

>T35385 NZ_AP022345:1586528-1586833 [Fluviibacter phosphoraccumulans]
ATGAAACTTATCTATACGACAGAAATTTTTGATGCTTGGTTTGGCCGATTAAAAGACAAAGAAGTTGCGCGGCGGATTCA
GATTCGGATCGACAGAGCAGAAGATGGTCATTTCGGAGACTGTAAGTCTGTTGGTGGCGGTGTTTCAGAAATGCGAATTC
ATCATGGGCCGGGATATCGGGTGTATTTCATGCAACGAGGATTCGAGGTGGTGATTTTACTAGCAGGTGGTGACAAGTCA
TCACAGTCCAGAGATATTCAAACAGCTATACATATAGCTCAGCAGATTAAGGGTAGCAGATCATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10721.36 Da
• Isoelectric Point: 7.2776

>AT35385 WP_162050013.1 NZ_AP022345:1586830-1587129 [Fluviibacter phosphoraccumulans]
MKAVKLRRWDSAEHLKTSEEMALYLEACFQEAGDDAAFIAKALGNIARAKGMSQLAQETGMGRESLYKALSGEGNPSFAT
ILKVTRALGIELHAMPVHA

Nucleotide

• Length: 300 bp

>AT35385 NZ_AP022345:1586830-1587129 [Fluviibacter phosphoraccumulans]
ATGAAAGCAGTAAAGCTACGTAGATGGGATAGCGCCGAACATCTGAAAACATCAGAAGAGATGGCGTTGTATCTAGAAGC
TTGCTTCCAAGAAGCAGGTGATGATGCTGCATTTATTGCCAAAGCACTGGGTAACATTGCACGTGCCAAAGGCATGTCAC
AGCTAGCCCAAGAGACCGGTATGGGACGGGAGAGTCTTTATAAAGCGCTCTCCGGAGAAGGTAACCCAAGCTTTGCAACC
ATTCTCAAGGTGACACGTGCTTTAGGTATTGAGCTTCATGCGATGCCAGTTCATGCCTAG