Detailed information of TA system TA035384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 1400914..1402352
• Replicon: chromosome
• Accession: NZ_AP022345
• Organism: Fluviibacter phosphoraccumulans strain ICHIAU1

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: ICHIAU1_RS07010
• Protein ID: WP_162050167.1
• Coordinates: 1400914..1401753 (-)
• Length: 280 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: ICHIAU1_RS07015
• Protein ID: WP_162050166.1
• Coordinates: 1401750..1402352 (-)
• Length: 201 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICHIAU1_RS06980	1396243..1396803	-	561	WP_162050173.1	hypothetical protein	-
ICHIAU1_RS06985	1396814..1398142	-	1329	WP_162050172.1	TolC family protein	-
ICHIAU1_RS06990	1398217..1398591	-	375	WP_162050171.1	hypothetical protein	-
ICHIAU1_RS06995	1398650..1399879	-	1230	WP_162050170.1	IS256 family transposase	-
ICHIAU1_RS07000	1400233..1400538	+	306	WP_162050169.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ICHIAU1_RS07005	1400531..1400848	+	318	WP_162050168.1	putative addiction module antidote protein	-
ICHIAU1_RS07010	1400914..1401753	-	840	WP_162050167.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ICHIAU1_RS07015	1401750..1402352	-	603	WP_162050166.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
ICHIAU1_RS07020	1402442..1403464	-	1023	WP_162050165.1	hypothetical protein	-
ICHIAU1_RS07025	1403918..1404061	+	144	WP_162071257.1	hypothetical protein	-
ICHIAU1_RS07030	1404158..1405372	+	1215	WP_162071258.1	hypothetical protein	-
ICHIAU1_RS07035	1405478..1406077	-	600	WP_162071259.1	class I SAM-dependent methyltransferase	-
ICHIAU1_RS07040	1406090..1406476	-	387	WP_162071260.1	rhodanese-like domain-containing protein	-
ICHIAU1_RS07045	1406480..1407010	-	531	WP_162071261.1	YeeE/YedE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1327901..1403740	75839
	-	flank	IS/Tn	-	-	1398650..1399879	1229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (17-53)

Sequences

Toxin

Protein

• Length: 280 a.a.
• Molecular weight: 31236.08 Da
• Isoelectric Point: 4.5766

>T35384 WP_162050167.1 NZ_AP022345:c1401753-1400914 [Fluviibacter phosphoraccumulans]
MINRAASVRARLLAKAKAESQDFSLVLTRYGLERLLYRLSVSSNKDNFLLKGALLFDLWFDVPLRPTRDIDLLGFGPAEE
PLVLGAFQEICQIECDDGIEFDLDTLKIEEIRKDANYSGLRISLTGYVDGARCPIQVDIGYGDAVTPAPETAEYPVMLPD
TPVPILRVYPRYTVIAEKLEALIFLGMINTRLKDYFDLWVILTTTELDQNLLGEAITATLVRRKTALPASMPVGLSEEFS
SDAQKIAQWNAFVKKNQLNAPSLDVVVTILRDRFAQFVS

Nucleotide

• Length: 840 bp

>T35384 NZ_AP022345:c1401753-1400914 [Fluviibacter phosphoraccumulans]
ATGATCAATCGTGCCGCATCTGTTCGCGCTCGTTTGTTGGCCAAAGCCAAGGCCGAGAGCCAGGATTTCTCATTGGTGCT
AACCCGCTACGGACTTGAGCGTCTGCTATACCGTTTGAGCGTGTCTTCGAACAAAGACAACTTTTTACTCAAGGGTGCTC
TGCTGTTCGATTTGTGGTTCGACGTGCCGCTACGCCCCACCCGCGATATCGATTTGCTTGGGTTTGGTCCAGCCGAAGAG
CCTTTGGTATTGGGAGCGTTTCAGGAGATATGCCAGATTGAGTGCGACGATGGCATTGAGTTTGATCTCGACACCCTGAA
AATCGAGGAAATTCGAAAGGACGCCAATTATTCCGGGCTACGTATCAGTTTGACTGGATACGTCGATGGGGCACGCTGCC
CCATCCAGGTCGACATCGGCTACGGTGACGCCGTAACGCCAGCGCCTGAAACAGCGGAATATCCAGTCATGTTGCCGGAC
ACCCCAGTGCCGATACTTCGGGTCTATCCTCGGTATACGGTCATCGCTGAGAAATTAGAAGCACTGATCTTTCTGGGAAT
GATCAATACCCGACTTAAGGACTATTTCGACTTATGGGTCATTCTCACTACTACTGAGTTAGACCAAAACTTACTCGGTG
AAGCTATTACCGCAACCTTGGTTCGCAGAAAAACGGCGCTTCCAGCATCGATGCCCGTGGGCCTGTCAGAGGAGTTTTCT
TCTGATGCCCAGAAGATCGCCCAGTGGAACGCATTCGTTAAGAAAAACCAGCTCAATGCGCCGAGCCTTGATGTCGTTGT
TACCATTTTGCGCGACAGGTTCGCTCAATTTGTAAGTTAA

Antitoxin

Protein

• Length: 201 a.a.
• Molecular weight: 22378.14 Da
• Isoelectric Point: 10.3044

>AT35384 WP_162050166.1 NZ_AP022345:c1402352-1401750 [Fluviibacter phosphoraccumulans]
MKRQAHPVHQLLTQKTLLRPKDAVSSGYSRIYLAQLAKRGALRKVGRGLYASPLRKESEESGLAEVAMKYPRAVVCLLSA
LSVHGLTTQAPHEVWVAVGSKDRAPKMDYPPLRVVRFGGETLTEGSEVKVVEGIPVQITNVPKTIADCFKFRNKIGLDVA
LEALKEAWRDKRVSIDDLWRYAQLCRVQNVMRPYLESLVA

Nucleotide

• Length: 603 bp

>AT35384 NZ_AP022345:c1402352-1401750 [Fluviibacter phosphoraccumulans]
ATGAAAAGGCAAGCACACCCCGTCCACCAGCTACTGACGCAAAAGACGCTACTCCGTCCCAAAGACGCGGTATCAAGCGG
CTATTCGCGTATTTATCTGGCTCAGCTGGCCAAACGTGGTGCACTACGTAAGGTCGGCCGAGGGCTGTATGCCTCCCCGC
TGCGCAAAGAGTCAGAAGAGTCCGGCCTCGCCGAAGTGGCCATGAAATATCCACGGGCCGTTGTCTGCCTGCTGTCTGCC
CTGTCTGTTCATGGGCTCACAACCCAGGCACCCCACGAAGTATGGGTGGCCGTGGGTAGTAAGGATCGCGCCCCAAAAAT
GGATTATCCACCTCTCCGAGTCGTTCGCTTCGGAGGAGAAACACTAACAGAGGGGTCCGAGGTGAAGGTGGTTGAGGGCA
TCCCTGTTCAAATCACCAATGTTCCCAAGACGATTGCAGATTGCTTCAAGTTCCGCAACAAGATTGGCCTGGACGTAGCA
CTGGAAGCGCTGAAGGAGGCTTGGCGGGATAAGCGCGTCAGCATCGATGATCTGTGGCGTTACGCACAGCTATGTCGCGT
ACAGAATGTCATGCGCCCCTACCTTGAAAGCCTGGTCGCATGA