Detailed information of TA system TA035383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1400233..1400848
• Replicon: chromosome
• Accession: NZ_AP022345
• Organism: Fluviibacter phosphoraccumulans strain ICHIAU1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ICHIAU1_RS07000
• Protein ID: WP_162050169.1
• Coordinates: 1400233..1400538 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ICHIAU1_RS07005
• Protein ID: WP_162050168.1
• Coordinates: 1400531..1400848 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICHIAU1_RS06980	1396243..1396803	-	561	WP_162050173.1	hypothetical protein	-
ICHIAU1_RS06985	1396814..1398142	-	1329	WP_162050172.1	TolC family protein	-
ICHIAU1_RS06990	1398217..1398591	-	375	WP_162050171.1	hypothetical protein	-
ICHIAU1_RS06995	1398650..1399879	-	1230	WP_162050170.1	IS256 family transposase	-
ICHIAU1_RS07000	1400233..1400538	+	306	WP_162050169.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICHIAU1_RS07005	1400531..1400848	+	318	WP_162050168.1	putative addiction module antidote protein	Antitoxin
ICHIAU1_RS07010	1400914..1401753	-	840	WP_162050167.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
ICHIAU1_RS07015	1401750..1402352	-	603	WP_162050166.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
ICHIAU1_RS07020	1402442..1403464	-	1023	WP_162050165.1	hypothetical protein	-
ICHIAU1_RS07025	1403918..1404061	+	144	WP_162071257.1	hypothetical protein	-
ICHIAU1_RS07030	1404158..1405372	+	1215	WP_162071258.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1327901..1403740	75839
	-	flank	IS/Tn	-	-	1398650..1399879	1229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-85)

Antitoxin

• Cro/C1-type helix-turn-helix domain (51-94)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11471.35 Da
• Isoelectric Point: 9.9485

>T35383 WP_162050169.1 NZ_AP022345:1400233-1400538 [Fluviibacter phosphoraccumulans]
MYTVIQIPAFSDWLRAIKDRQTQVRLMKRLRKAQLGNLGDVKQVGSGVWEMREFFGPGWRMYYVQRGDLLIVMLGGGDKS
TQTADIAKAISLASLVEIEHD

Nucleotide

• Length: 306 bp

>T35383 NZ_AP022345:1400233-1400538 [Fluviibacter phosphoraccumulans]
ATGTACACCGTTATACAAATCCCGGCCTTTTCTGATTGGCTCCGAGCCATTAAAGATCGCCAGACACAGGTTCGTCTAAT
GAAGCGCCTTCGTAAAGCGCAATTAGGTAATCTGGGGGATGTTAAACAGGTGGGCAGTGGTGTTTGGGAGATGCGGGAGT
TTTTCGGCCCCGGCTGGCGCATGTACTACGTTCAGCGTGGGGATTTGTTGATCGTGATGCTGGGTGGTGGAGATAAATCT
ACTCAAACAGCAGACATTGCTAAAGCCATTTCTCTTGCGAGCCTGGTGGAGATTGAACATGACTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11516.30 Da
• Isoelectric Point: 5.1568

>AT35383 WP_162050168.1 NZ_AP022345:1400531-1400848 [Fluviibacter phosphoraccumulans]
MTKKIKLADCRPFDIAEYLDDETMITEYMNVVLEENDPAMLASALGDIARARGMSQLAKETGLSRESLYKGLSGDRIPST
ETLLKVIHALGFKLSVSKVDTVAHA

Nucleotide

• Length: 318 bp

>AT35383 NZ_AP022345:1400531-1400848 [Fluviibacter phosphoraccumulans]
ATGACTAAGAAGATTAAACTTGCCGATTGCCGACCATTTGATATTGCAGAATATCTGGACGACGAAACCATGATTACAGA
GTATATGAACGTTGTGCTTGAAGAGAACGACCCAGCGATGTTGGCTTCAGCCTTGGGCGATATCGCTCGTGCTCGTGGCA
TGTCACAACTTGCGAAAGAGACTGGCCTCAGCAGAGAAAGCTTATACAAGGGGTTGTCTGGTGACCGTATCCCAAGTACC
GAGACCCTGCTAAAAGTCATTCATGCGCTGGGATTCAAGCTATCAGTATCCAAGGTGGATACAGTCGCGCACGCTTAA