Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 547782..548353 Replicon chromosome
Accession NZ_AP022341
Organism Enterococcus faecium strain KUHS13

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag EfmKUHS13_RS02615 Protein ID WP_002286801.1
Coordinates 548012..548353 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag EfmKUHS13_RS02610 Protein ID WP_002323011.1
Coordinates 547782..548012 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EfmKUHS13_RS02585 543263..544591 + 1329 WP_002327434.1 FAD-containing oxidoreductase -
EfmKUHS13_RS02590 544613..545239 + 627 WP_002352507.1 cysteine hydrolase -
EfmKUHS13_RS02595 545422..546003 + 582 WP_002327433.1 TetR/AcrR family transcriptional regulator -
EfmKUHS13_RS02600 546368..546943 + 576 WP_002352505.1 SOS response-associated peptidase family protein -
EfmKUHS13_RS02605 547148..547486 - 339 WP_002306002.1 hypothetical protein -
EfmKUHS13_RS02610 547782..548012 + 231 WP_002323011.1 hypothetical protein Antitoxin
EfmKUHS13_RS02615 548012..548353 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EfmKUHS13_RS02620 548688..549145 + 458 Protein_522 transposase -
EfmKUHS13_RS02625 549240..550199 + 960 WP_002301399.1 IS30 family transposase -
EfmKUHS13_RS02630 550205..550916 + 712 Protein_524 IS3-like element IS1485 family transposase -
EfmKUHS13_RS02635 551164..551289 + 126 WP_025478972.1 LPXTG cell wall anchor domain-containing protein -
EfmKUHS13_RS02640 551363..552259 + 897 WP_002352497.1 class C sortase -
EfmKUHS13_RS02645 552440..553114 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 546368..559683 13315


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T35373 WP_002286801.1 NZ_AP022341:548012-548353 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T35373 NZ_AP022341:548012-548353 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT35373 WP_002323011.1 NZ_AP022341:547782-548012 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT35373 NZ_AP022341:547782-548012 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References