Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 222343..222872 Replicon chromosome
Accession NZ_AP022341
Organism Enterococcus faecium strain KUHS13

Toxin (Protein)


Gene name relE Uniprot ID Q3XX67
Locus tag EfmKUHS13_RS01095 Protein ID WP_002296560.1
Coordinates 222606..222872 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A829F3T0
Locus tag EfmKUHS13_RS01090 Protein ID WP_002296559.1
Coordinates 222343..222606 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EfmKUHS13_RS01075 219717..221540 + 1824 WP_002294202.1 APC family permease -
EfmKUHS13_RS01080 221682..221867 - 186 WP_002296558.1 transcriptional regulator -
EfmKUHS13_RS01090 222343..222606 + 264 WP_002296559.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EfmKUHS13_RS01095 222606..222872 + 267 WP_002296560.1 Txe/YoeB family addiction module toxin Toxin
EfmKUHS13_RS01100 223073..223561 + 489 WP_002296561.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
EfmKUHS13_RS01105 224102..224773 - 672 WP_002302663.1 ABC transporter permease -
EfmKUHS13_RS01110 224770..225687 - 918 WP_002296564.1 osmoprotectant ABC transporter substrate-binding protein -
EfmKUHS13_RS01115 225684..226325 - 642 WP_002293405.1 ABC transporter permease -
EfmKUHS13_RS01120 226329..226910 - 582 Protein_223 CBS domain-containing protein -
EfmKUHS13_RS01125 227068..227472 + 405 WP_002287522.1 IS200/IS605-like element ISEfa4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element tet(M) / tet(L) lap 120492..320783 200291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10660.13 Da        Isoelectric Point: 9.9931

>T35372 WP_002296560.1 NZ_AP022341:222606-222872 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T35372 NZ_AP022341:222606-222872 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10046.05 Da        Isoelectric Point: 4.1207

>AT35372 WP_002296559.1 NZ_AP022341:222343-222606 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNID
DIDWEAL

Download         Length: 264 bp

>AT35372 NZ_AP022341:222343-222606 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FCW8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3T0

References