Detailed information of TA system TA035371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 125019..126043
• Replicon: plasmid pKUB257
• Accession: NZ_AP022338
• Organism: Mameliella alba strain KU6B

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: KU6B_RS27385
• Protein ID: WP_172431608.1
• Coordinates: 125480..126043 (+)
• Length: 188 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: KU6B_RS27380
• Protein ID: WP_172431607.1
• Coordinates: 125019..125483 (+)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU6B_RS27355	120778..121143	+	366	Protein_126	transposase	-
KU6B_RS27360	121392..121610	+	219	Protein_127	hypothetical protein	-
KU6B_RS27365	121748..122803	-	1056	WP_172431604.1	tyrosine-type recombinase/integrase	-
KU6B_RS27370	123102..123986	+	885	WP_172431605.1	DUF1403 family protein	-
KU6B_RS27375	123989..124603	+	615	WP_172431606.1	SMC-Scp complex subunit ScpB	-
KU6B_RS27380	125019..125483	+	465	WP_172431607.1	helix-turn-helix domain-containing protein	Antitoxin
KU6B_RS27385	125480..126043	+	564	WP_172431608.1	PIN domain-containing protein	Toxin
KU6B_RS27390	126096..126812	-	717	WP_172431609.1	DUF45 domain-containing protein	-
KU6B_RS27395	126809..130033	-	3225	WP_172431610.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..256516	256516

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (11-115)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 188 a.a.
• Molecular weight: 20458.23 Da
• Isoelectric Point: 4.3246

>T35371 WP_172431608.1 NZ_AP022338:125480-126043 [Mameliella alba]
MSRYADRFTALLDACVLGGALRRNMLLSLAEAGLFRPRWSSRILDETQKAITQITKGETDGSRQRAAIEVAFPEALVTGY
EMFEDKLALPDPNDNHVLAAAIVTSASVIVTDNLADFPSAVLDPHAIQAVSADDFIADTIELDPSEAILALRRMRERFKN
PALDVAASEAQGLLQVATFMDEYQSFL

Nucleotide

• Length: 564 bp

>T35371 NZ_AP022338:125480-126043 [Mameliella alba]
ATGAGCCGATACGCTGATCGGTTCACGGCTCTCCTCGATGCCTGCGTCCTGGGCGGAGCGCTTCGCAGAAACATGCTCCT
GAGCCTGGCCGAGGCCGGCTTGTTCCGGCCTCGGTGGAGCAGTCGCATTCTTGACGAGACCCAGAAAGCGATCACCCAGA
TCACCAAGGGCGAAACCGATGGGTCCCGGCAAAGGGCGGCAATCGAAGTTGCATTCCCTGAAGCTCTGGTGACTGGTTAT
GAGATGTTCGAAGACAAGCTCGCGTTGCCCGACCCCAACGACAACCACGTACTGGCAGCGGCCATCGTGACGTCTGCTTC
AGTCATCGTGACGGACAATCTTGCCGACTTTCCTTCCGCCGTTCTGGACCCGCACGCCATCCAGGCGGTTTCCGCCGACG
ACTTCATTGCCGATACAATCGAGCTGGATCCCTCGGAAGCCATTCTGGCCCTGCGTCGCATGCGGGAGCGCTTCAAGAAC
CCCGCGCTCGATGTTGCGGCGTCAGAAGCCCAGGGCCTATTGCAGGTTGCAACCTTCATGGACGAGTACCAGTCGTTCCT
GTAG

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 17080.55 Da
• Isoelectric Point: 4.9258

>AT35371 WP_172431607.1 NZ_AP022338:125019-125483 [Mameliella alba]
MNALRKEAFFDRLPDEVEIKNAEQLRTIVASQIKEGEPTKLSLALEGGEVRTVTLAPALTASLLEVLRLVSSGRGFRMIP
VESELTTQQAADLLNVSRPFLVKLLEEGEIPFAKTGRHRRVRADDLFAYKEKRDASRSDALSDLATMDAEEGLI

Nucleotide

• Length: 465 bp

>AT35371 NZ_AP022338:125019-125483 [Mameliella alba]
ATGAACGCCCTCAGAAAAGAGGCGTTCTTTGATCGCTTGCCCGACGAGGTCGAGATCAAGAACGCCGAACAACTCAGAAC
GATTGTCGCCTCCCAGATCAAGGAAGGCGAACCGACAAAGCTGTCGCTCGCCCTGGAAGGCGGCGAAGTTCGCACCGTGA
CGCTTGCGCCGGCCTTGACGGCTTCGCTACTCGAAGTGCTGCGGCTCGTGTCGAGTGGTCGCGGGTTTCGGATGATCCCC
GTCGAGTCAGAGCTGACCACCCAGCAGGCCGCGGACCTTCTCAATGTGTCCCGTCCGTTCCTGGTGAAGCTTCTCGAGGA
AGGCGAGATTCCGTTTGCGAAAACCGGCCGTCATCGCCGGGTTCGTGCCGACGATCTCTTTGCCTACAAGGAGAAGCGTG
ACGCGTCTCGCTCGGACGCTTTGAGCGACCTTGCCACAATGGATGCGGAAGAAGGTCTTATATGA