Detailed information of TA system TA035369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 8352..8947
• Replicon: plasmid pMSC49d
• Accession: NZ_AP022336
• Organism: Methylosinus sp. C49 isolate Methylosinus sp. C49

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GYH34_RS21555
• Protein ID: WP_142863163.1
• Coordinates: 8352..8645 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GYH34_RS21560
• Protein ID: WP_142863162.1
• Coordinates: 8645..8947 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYH34_RS21525	3471..3791	+	321	WP_161915600.1	mobilization protein	-
GYH34_RS21530	3883..4149	+	267	WP_161915601.1	hypothetical protein	-
GYH34_RS21705	4223..4426	+	204	WP_162561596.1	hypothetical protein	-
GYH34_RS21535	4772..5275	-	504	WP_161915602.1	hypothetical protein	-
GYH34_RS21540	5444..5701	+	258	WP_161915603.1	helix-turn-helix domain-containing protein	-
GYH34_RS21545	5817..6587	-	771	WP_161915604.1	hypothetical protein	-
GYH34_RS21550	7551..8192	-	642	WP_142863164.1	ParA family protein	-
GYH34_RS21555	8352..8645	+	294	WP_142863163.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GYH34_RS21560	8645..8947	+	303	WP_142863162.1	putative addiction module antidote protein	Antitoxin
GYH34_RS21565	8956..9507	-	552	WP_161915605.1	recombinase family protein	-
GYH34_RS21570	9641..10321	-	681	WP_161915606.1	hypothetical protein	-
GYH34_RS21575	10572..11333	+	762	WP_161915607.1	helix-turn-helix domain-containing protein	-
GYH34_RS21580	11720..12205	+	486	WP_161915608.1	helix-turn-helix domain-containing protein	-
GYH34_RS21590	12821..13696	+	876	WP_161915609.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..22482	22482

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-90)

Antitoxin

• Uncharacterized protein HI1420 (6-97)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10983.78 Da
• Isoelectric Point: 10.1348

>T35369 WP_142863163.1 NZ_AP022336:8352-8645 [Methylosinus sp. C49]
MLKIRQTLVFQEWRESLKDGRARAAIAKRMIRIQAGNFGDAKYLGDGVSELRFDLGPGYRVYFHRRGDVVVILLCGGDKK
TQASDIEKAKAMIPELE

Nucleotide

• Length: 294 bp

>T35369 NZ_AP022336:8352-8645 [Methylosinus sp. C49]
ATGCTCAAAATCCGCCAGACACTTGTGTTTCAAGAATGGCGGGAATCTCTCAAAGACGGGCGCGCAAGAGCCGCCATAGC
GAAGCGGATGATCCGCATCCAAGCCGGGAATTTTGGAGATGCCAAATATCTCGGCGATGGCGTCAGCGAGCTGAGGTTCG
ACCTTGGACCGGGATATCGGGTTTATTTCCACCGTCGCGGCGACGTCGTGGTGATTTTGCTCTGCGGCGGTGACAAAAAA
ACGCAAGCGTCCGACATCGAAAAGGCCAAGGCCATGATCCCAGAACTGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10452.96 Da
• Isoelectric Point: 4.5388

>AT35369 WP_142863162.1 NZ_AP022336:8645-8947 [Methylosinus sp. C49]
MTVPIKTIPYDPAEDLTDDKDQVELLAEAFSSGDQKYIAAALGAVARARGMSTIAEKAGVTRPALYKALSETGDPQLSTL
LGVVNALGLKLAVEPLAHTT

Nucleotide

• Length: 303 bp

>AT35369 NZ_AP022336:8645-8947 [Methylosinus sp. C49]
ATGACCGTGCCGATCAAAACAATTCCCTATGATCCCGCTGAAGACCTAACTGACGACAAGGATCAAGTCGAACTGCTGGC
GGAAGCTTTTTCTTCTGGCGATCAAAAATACATCGCGGCCGCTCTTGGCGCGGTCGCGCGAGCACGTGGCATGTCCACAA
TCGCCGAAAAGGCCGGCGTCACACGCCCGGCTCTCTACAAAGCCCTAAGTGAAACTGGCGACCCACAACTCTCGACATTG
CTCGGCGTCGTCAATGCGCTTGGGCTAAAACTGGCGGTGGAGCCTCTGGCTCACACCACCTGA