Detailed information of TA system TA035368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 244223..244896
• Replicon: plasmid pMSC49b
• Accession: NZ_AP022334
• Organism: Methylosinus sp. C49 isolate Methylosinus sp. C49

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GYH34_RS20630
• Protein ID: WP_161915435.1
• Coordinates: 244223..244645 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GYH34_RS20635
• Protein ID: WP_161915436.1
• Coordinates: 244642..244896 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYH34_RS20620	240257..241243	-	987	WP_161915433.1	plasmid partitioning protein RepB	-
GYH34_RS20625	241240..242463	-	1224	WP_161915434.1	plasmid partitioning protein RepA	-
GYH34_RS20630	244223..244645	-	423	WP_161915435.1	type II toxin-antitoxin system VapC family toxin	Toxin
GYH34_RS20635	244642..244896	-	255	WP_161915436.1	plasmid stabilization protein	Antitoxin
GYH34_RS20640	245073..245780	+	708	WP_161915437.1	hypothetical protein	-
GYH34_RS20645	246952..247356	+	405	WP_161915438.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..263145	263145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14934.13 Da
• Isoelectric Point: 5.8928

>T35368 WP_161915435.1 NZ_AP022334:c244645-244223 [Methylosinus sp. C49]
MILLDTNVVSEAMKPEPDPAVRRWLDEQAAETLYLSSVTIAELMFGVGALPKGKRKDRLTAALEGVLELFADRILPFETN
AARRYSDLAVKARAAGKGFPTPDGYIAAIAASHGFVVASRDRSAFTAAGLTVLDPWSAGA

Nucleotide

• Length: 423 bp

>T35368 NZ_AP022334:c244645-244223 [Methylosinus sp. C49]
ATGATCCTGCTCGACACCAATGTCGTCTCCGAGGCGATGAAGCCCGAACCGGACCCGGCGGTCCGCCGCTGGCTCGATGA
GCAGGCGGCGGAAACGCTCTATCTCTCGAGCGTCACGATCGCTGAGCTGATGTTCGGCGTCGGCGCGCTTCCCAAGGGCA
AGCGCAAGGACAGGTTGACCGCCGCGCTCGAAGGCGTGCTGGAGCTGTTCGCGGACCGCATCCTCCCGTTCGAGACGAAC
GCGGCGCGCCGCTATTCCGATCTGGCGGTGAAGGCGCGCGCTGCCGGAAAGGGGTTCCCCACACCGGATGGCTATATCGC
CGCCATTGCCGCATCGCATGGTTTCGTCGTTGCATCCCGCGACCGCAGCGCATTCACTGCCGCCGGTCTAACCGTGCTCG
ACCCCTGGAGCGCCGGGGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9385.65 Da
• Isoelectric Point: 8.9326

>AT35368 WP_161915436.1 NZ_AP022334:c244896-244642 [Methylosinus sp. C49]
MPAVTIRNLSEETHRALKVRAAQHGRSTEAEIRDILENAVRPANRLRLGSGLAEMSRKIGLTNADVEALEQAREQKPAEP
LRFE

Nucleotide

• Length: 255 bp

>AT35368 NZ_AP022334:c244896-244642 [Methylosinus sp. C49]
ATGCCTGCTGTCACCATCCGCAACCTCTCCGAAGAGACCCATCGCGCGCTGAAGGTCCGTGCGGCGCAGCACGGACGCAG
CACGGAAGCGGAGATACGCGACATCCTGGAGAACGCTGTGCGCCCGGCCAATCGTCTCCGCCTTGGAAGCGGGCTGGCGG
AAATGAGCCGGAAGATCGGCCTGACCAACGCCGACGTCGAAGCCCTCGAACAGGCCCGCGAGCAGAAGCCGGCAGAGCCG
CTGCGCTTCGAATGA