Detailed information of TA system TA035367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 151027..151700
• Replicon: plasmid pMSC49a
• Accession: NZ_AP022333
• Organism: Methylosinus sp. C49 isolate Methylosinus sp. C49

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GYH34_RS19085
• Protein ID: WP_161915196.1
• Coordinates: 151278..151700 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GYH34_RS19080
• Protein ID: WP_161915195.1
• Coordinates: 151027..151281 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYH34_RS19055	146607..148055	+	1449	WP_161915191.1	HAMP domain-containing histidine kinase	-
GYH34_RS19060	148135..148566	-	432	WP_161915192.1	DoxX family protein	-
GYH34_RS19065	148613..149311	-	699	WP_161915193.1	pirin family protein	-
GYH34_RS19070	149947..150279	+	333	WP_161915194.1	hypothetical protein	-
GYH34_RS19075	150665..150787	-	123	Protein_124	DJ-1/PfpI family protein	-
GYH34_RS19080	151027..151281	+	255	WP_161915195.1	plasmid stabilization protein	Antitoxin
GYH34_RS19085	151278..151700	+	423	WP_161915196.1	type II toxin-antitoxin system VapC family toxin	Toxin
GYH34_RS19090	152286..153440	-	1155	WP_161915197.1	SRPBCC domain-containing protein	-
GYH34_RS19095	153437..155065	-	1629	Protein_128	MFS transporter	-
GYH34_RS19100	155192..155539	-	348	WP_161915198.1	metalloregulator ArsR/SmtB family transcription factor	-
GYH34_RS19105	156193..156618	+	426	WP_161915199.1	putative toxin-antitoxin system toxin component, PIN family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..324889	324889

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14874.08 Da
• Isoelectric Point: 5.8928

>T35367 WP_161915196.1 NZ_AP022333:151278-151700 [Methylosinus sp. C49]
MILLDTNVVSEAMKPEPDPAVRRWLDEQAAETLYLSSVTIAELMFGVGALPKGKRKDRLTAALEGVLELFADRILPFETN
AARRYSDLAVKARAAGKGFPTPDGYIAAIAASHGFVVASRDRSAFAAAGLSVIDPWAAGA

Nucleotide

• Length: 423 bp

>T35367 NZ_AP022333:151278-151700 [Methylosinus sp. C49]
ATGATCCTGCTCGACACCAATGTCGTCTCCGAGGCGATGAAGCCCGAACCGGACCCGGCGGTCCGCCGATGGCTCGATGA
GCAGGCGGCGGAAACGCTCTATCTCTCGAGCGTCACGATCGCTGAGCTGATGTTCGGCGTCGGCGCGCTTCCCAAGGGCA
AGCGCAAGGACAGGTTGACCGCCGCGCTCGAAGGCGTGCTGGAGCTGTTCGCGGACCGCATCCTCCCGTTCGAGACGAAC
GCGGCGCGCCGCTATTCCGATCTGGCGGTGAAGGCGCGCGCTGCCGGAAAGGGGTTCCCCACACCGGATGGCTATATCGC
CGCCATTGCCGCATCGCATGGTTTCGTCGTTGCATCCCGCGACCGCAGCGCATTCGCGGCCGCCGGCCTGAGCGTGATCG
ACCCCTGGGCCGCCGGGGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9399.68 Da
• Isoelectric Point: 8.9326

>AT35367 WP_161915195.1 NZ_AP022333:151027-151281 [Methylosinus sp. C49]
MPAVTIRNLSEETHRALKVRAAQHGRSTEAEIRDILENAVRPANRLRLGSALAEMSRKIGLTNADVEALEQAREQKPAEP
LRFE

Nucleotide

• Length: 255 bp

>AT35367 NZ_AP022333:151027-151281 [Methylosinus sp. C49]
ATGCCTGCTGTCACCATCCGCAACCTCTCCGAAGAGACGCATCGCGCGCTGAAGGTCCGTGCGGCGCAGCACGGACGCAG
CACGGAAGCGGAGATACGCGACATCCTGGAGAACGCTGTGCGCCCGGCCAATCGTCTCCGCCTTGGAAGCGCGCTGGCGG
AAATGAGCCGGAAGATCGGCCTGACCAACGCCGACGTCGAAGCCCTCGAACAGGCCCGCGAGCAGAAGCCGGCAGAACCG
CTGCGCTTCGAATGA