Detailed information of TA system TA035365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1503637..1504447
• Replicon: chromosome
• Accession: NZ_AP022332
• Organism: Methylosinus sp. C49 isolate Methylosinus sp. C49

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GYH34_RS07135
• Protein ID: WP_161912962.1
• Coordinates: 1503637..1504164 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: GYH34_RS07140
• Protein ID: WP_161912963.1
• Coordinates: 1504157..1504447 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYH34_RS07115	1498793..1499791	+	999	WP_161912959.1	LLM class flavin-dependent oxidoreductase	-
GYH34_RS07120	1499876..1500655	-	780	WP_161912960.1	hypothetical protein	-
GYH34_RS07125	1500802..1501506	+	705	WP_064033743.1	transcriptional repressor LexA	-
GYH34_RS07130	1501720..1503627	+	1908	WP_161912961.1	AsmA family protein	-
GYH34_RS07135	1503637..1504164	-	528	WP_161912962.1	GNAT family N-acetyltransferase	Toxin
GYH34_RS07140	1504157..1504447	-	291	WP_161912963.1	DUF1778 domain-containing protein	Antitoxin
GYH34_RS07145	1504497..1505291	-	795	WP_161912964.1	ABC transporter permease subunit	-
GYH34_RS07150	1505288..1506205	-	918	WP_161912965.1	ABC transporter permease subunit	-
GYH34_RS07155	1506202..1507260	-	1059	WP_161912966.1	ABC transporter ATP-binding protein	-
GYH34_RS07160	1507316..1508026	-	711	WP_161912967.1	class I SAM-dependent methyltransferase	-
GYH34_RS07165	1508008..1508475	-	468	WP_161912968.1	DNA polymerase III subunit chi	-
GYH34_RS07170	1508513..1509289	-	777	WP_161912969.1	exodeoxyribonuclease III	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (34-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-89)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19219.28 Da
• Isoelectric Point: 9.7099

>T35365 WP_161912962.1 NZ_AP022332:c1504164-1503637 [Methylosinus sp. C49]
MAETPPSLEPPAPLSESHELAEFRSGETVLDEWLMRRALANMEAGASKTYVLCAAGSQQVVGFYALSMGQILDREALGSM
RRNMPRHIPSVILGRLAIDARCQGRGIGRALLHDAVSRSRRAAREVAARLLVVHAISPTAEAFYRHHGFMRLPVETPTLA
LDLVKLERVIGKERG

Nucleotide

• Length: 528 bp

>T35365 NZ_AP022332:c1504164-1503637 [Methylosinus sp. C49]
ATGGCTGAAACGCCGCCGTCTCTCGAGCCGCCCGCGCCACTGAGCGAAAGCCATGAGCTCGCAGAATTTCGCAGCGGCGA
AACCGTTCTGGACGAATGGCTGATGAGGCGCGCCCTCGCCAATATGGAGGCCGGCGCGAGCAAGACCTATGTGCTGTGCG
CCGCCGGCTCGCAACAGGTCGTCGGCTTCTATGCGCTGAGCATGGGCCAGATTCTCGATCGGGAAGCGCTCGGCTCCATG
CGGCGAAACATGCCGCGACATATTCCTTCGGTGATCCTCGGGCGGCTCGCCATAGACGCGCGCTGTCAGGGACGCGGCAT
AGGCAGAGCGCTGCTTCACGACGCCGTTTCACGCTCGCGGCGCGCCGCGCGCGAAGTGGCCGCCCGCCTCCTCGTCGTGC
ACGCGATCTCGCCGACGGCGGAGGCCTTCTACCGCCATCATGGCTTCATGCGCCTGCCGGTGGAAACGCCGACGCTGGCG
CTCGATCTCGTGAAGCTCGAAAGGGTGATCGGAAAAGAGAGAGGCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10744.25 Da
• Isoelectric Point: 10.2844

>AT35365 WP_161912963.1 NZ_AP022332:c1504447-1504157 [Methylosinus sp. C49]
MKPAPPLAEVNIHLRAREQDRSLIDQAAELTGTNRSQFMLASALSAAKNVLLDQTTIVADRRAFRKILDWMDAPASDEEE
AGMKRLAARRAQWRNG

Nucleotide

• Length: 291 bp

>AT35365 NZ_AP022332:c1504447-1504157 [Methylosinus sp. C49]
ATGAAACCCGCGCCGCCGCTCGCCGAAGTCAATATTCACCTGCGCGCCCGCGAGCAGGACCGCTCGCTCATCGACCAGGC
GGCCGAATTGACGGGAACCAATCGCTCGCAATTCATGCTCGCCTCCGCCTTGAGCGCGGCGAAGAATGTCCTGCTCGATC
AGACGACCATCGTGGCGGACCGGCGAGCCTTTCGAAAAATTCTCGATTGGATGGACGCCCCGGCTTCCGACGAGGAAGAA
GCAGGCATGAAGCGGCTCGCCGCGCGCAGAGCCCAATGGCGGAATGGCTGA