Detailed information of TA system TA035362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 190141..190715
• Replicon: chromosome
• Accession: NZ_AP022332
• Organism: Methylosinus sp. C49 isolate Methylosinus sp. C49

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GYH34_RS00885
• Protein ID: WP_161911947.1
• Coordinates: 190141..190473 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GYH34_RS00890
• Protein ID: WP_161911948.1
• Coordinates: 190473..190715 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYH34_RS00865	185529..187364	+	1836	WP_161911944.1	membrane protein insertase YidC	-
GYH34_RS00870	187372..188034	+	663	WP_161911945.1	ribosome biogenesis GTP-binding protein YihA/YsxC	-
GYH34_RS00875	188093..188977	+	885	WP_142862633.1	acetylglutamate kinase	-
GYH34_RS00880	189071..189796	+	726	WP_161911946.1	pyrimidine 5'-nucleotidase	-
GYH34_RS00885	190141..190473	-	333	WP_161911947.1	endoribonuclease MazF	Toxin
GYH34_RS00890	190473..190715	-	243	WP_161911948.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GYH34_RS00895	190916..191290	+	375	WP_161911949.1	hypothetical protein	-
GYH34_RS00900	191349..193652	+	2304	WP_161911950.1	TonB-dependent siderophore receptor	-
GYH34_RS00905	193665..195212	+	1548	WP_161911951.1	PepSY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-105)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11747.72 Da
• Isoelectric Point: 10.2592

>T35362 WP_161911947.1 NZ_AP022332:c190473-190141 [Methylosinus sp. C49]
MAAYTPERGDIVWLSFDPQAGHEQAGHRPALVLSPNAYNAKTGLMICCPMTTKIKGYPFEVAIAGPTPSAALVDQVKSLD
WRARKAKKKGSASKSELAVATAKLRPLLGI

Nucleotide

• Length: 333 bp

>T35362 NZ_AP022332:c190473-190141 [Methylosinus sp. C49]
ATGGCGGCCTATACGCCCGAGCGTGGCGACATAGTATGGCTGAGCTTCGATCCACAGGCCGGCCATGAGCAAGCGGGGCA
TCGTCCCGCGCTCGTGTTGAGCCCGAATGCCTATAACGCCAAGACCGGGCTGATGATCTGCTGTCCGATGACGACGAAGA
TCAAGGGCTATCCCTTCGAGGTCGCGATCGCGGGACCGACGCCATCGGCGGCCCTCGTCGATCAGGTGAAAAGTCTGGAC
TGGCGCGCGCGCAAGGCGAAGAAGAAGGGCTCCGCCTCGAAGAGCGAGCTGGCGGTGGCAACGGCGAAGCTCAGGCCGCT
ATTGGGAATTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8627.79 Da
• Isoelectric Point: 4.2213

>AT35362 WP_161911948.1 NZ_AP022332:c190715-190473 [Methylosinus sp. C49]
MRAIVKKWGNSAAVRIPSAVMEQARLEIDQAVEVRVEGGSVVIEPLAAPEFSLEDLLAGVTSENLHEEESFGPPVGRETL

Nucleotide

• Length: 243 bp

>AT35362 NZ_AP022332:c190715-190473 [Methylosinus sp. C49]
ATGCGAGCGATCGTCAAAAAATGGGGCAACTCCGCGGCTGTGCGCATACCCTCGGCCGTCATGGAGCAGGCGCGCCTCGA
GATCGACCAAGCCGTCGAGGTGCGCGTCGAAGGCGGCAGCGTGGTGATAGAGCCGCTCGCGGCGCCCGAATTCTCTCTCG
AGGACCTTCTCGCAGGTGTGACGTCTGAAAACCTGCATGAGGAGGAGAGCTTCGGACCGCCTGTCGGCCGCGAGACGCTC
TAA