Detailed information of TA system TA035361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 111921..112504
• Replicon: chromosome
• Accession: NZ_AP022332
• Organism: Methylosinus sp. C49 isolate Methylosinus sp. C49

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A679FAM5
• Locus tag: GYH34_RS00515
• Protein ID: WP_018266890.1
• Coordinates: 112172..112504 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: GYH34_RS00510
• Protein ID: WP_161911887.1
• Coordinates: 111921..112172 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYH34_RS00490	107873..108565	+	693	WP_051465485.1	phosphorylase	-
GYH34_RS00495	108506..109657	-	1152	WP_161911884.1	adenosyl-hopene transferase HpnH	-
GYH34_RS00500	109894..110541	+	648	WP_161911885.1	phosphoribosylanthranilate isomerase	-
GYH34_RS00505	110615..111862	+	1248	WP_161911886.1	tryptophan synthase subunit beta	-
GYH34_RS00510	111921..112172	+	252	WP_161911887.1	antitoxin	Antitoxin
GYH34_RS00515	112172..112504	+	333	WP_018266890.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GYH34_RS00520	112557..113453	+	897	WP_161911888.1	tryptophan synthase subunit alpha	-
GYH34_RS00525	113450..114310	+	861	WP_161911889.1	acetyl-CoA carboxylase, carboxyltransferase subunit beta	-
GYH34_RS00530	114330..115637	+	1308	WP_161911890.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-
GYH34_RS00535	115722..116405	-	684	WP_161911891.1	type 1 glutamine amidotransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (5-82)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11848.53 Da
• Isoelectric Point: 8.2774

>T35361 WP_018266890.1 NZ_AP022332:112172-112504 [Methylosinus sp. C49]
MDRGDIYLVDLDPTEGREQKGRRCVMIVSASRFNRHTGAPLCVPITGGGGFARATGFTVPLTGSGLSTDGVVRCDQARTL
DLRARGARKLESAPDYIVNEVIARLTTLLE

Nucleotide

• Length: 333 bp

>T35361 NZ_AP022332:112172-112504 [Methylosinus sp. C49]
ATGGATCGAGGCGACATCTACCTCGTCGATCTCGATCCGACCGAAGGCCGTGAGCAAAAAGGCCGCCGCTGCGTGATGAT
CGTTTCGGCGAGCCGCTTCAACCGGCACACGGGGGCGCCGCTCTGCGTGCCGATCACCGGCGGGGGCGGCTTCGCTCGCG
CAACCGGCTTCACGGTTCCGCTCACGGGCAGCGGACTTTCGACCGATGGCGTCGTCCGCTGCGATCAGGCCCGGACGCTA
GATCTTCGCGCCCGCGGCGCCCGCAAGCTCGAGAGCGCTCCCGACTATATCGTCAATGAGGTGATCGCCCGGCTCACGAC
ATTGCTGGAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8954.26 Da
• Isoelectric Point: 4.3765

>AT35361 WP_161911887.1 NZ_AP022332:111921-112172 [Methylosinus sp. C49]
MQTAKLRKVGGSIMVAVPPAMLNALELAAESTVGLSVEGGRLIVEPQPHRRYSLDELLSEEAPSVSDQDEAWMNSPPIGR
ELL

Nucleotide

• Length: 252 bp

>AT35361 NZ_AP022332:111921-112172 [Methylosinus sp. C49]
ATGCAGACGGCCAAGCTACGAAAAGTCGGCGGCTCGATCATGGTGGCGGTTCCGCCCGCCATGCTCAACGCGCTCGAGCT
CGCGGCCGAATCGACCGTCGGCCTTTCGGTCGAGGGCGGGCGCCTGATCGTCGAGCCGCAACCCCATCGCCGCTATTCGC
TCGACGAGCTTTTGTCCGAAGAGGCCCCCTCTGTCTCCGACCAGGACGAAGCTTGGATGAATTCGCCGCCCATCGGCCGC
GAACTTCTCTGA

Structures

Toxin

• AlphaFold DB: A0A679FAM5