Detailed information of TA system TA035355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 130658..131259
• Replicon: plasmid p18044_1
• Accession: NZ_AP022327
• Organism: Escherichia coli O25:H4 strain 18044

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: ECO25NV_RS25270
• Protein ID: WP_001216034.1
• Coordinates: 130879..131259 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: ECO25NV_RS25265
• Protein ID: WP_001190712.1
• Coordinates: 130658..130879 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO25NV_RS25250	126405..127529	-	1125	Protein_149	HsdR family type I site-specific deoxyribonuclease	-
ECO25NV_RS25255	127651..128922	-	1272	WP_023142242.1	restriction endonuclease subunit S	-
ECO25NV_RS25260	128919..130475	-	1557	WP_001617892.1	type I restriction-modification system subunit M	-
ECO25NV_RS25265	130658..130879	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ECO25NV_RS25270	130879..131259	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ECO25NV_RS25275	131264..131443	+	180	WP_001513661.1	hypothetical protein	-
ECO25NV_RS25280	131471..131830	+	360	WP_001513660.1	hypothetical protein	-
ECO25NV_RS25285	131754..132167	+	414	Protein_156	DDE-type integrase/transposase/recombinase	-
ECO25NV_RS25290	132117..132434	-	318	WP_001513659.1	hypothetical protein	-
ECO25NV_RS25295	132662..133678	-	1017	WP_012372828.1	IS5-like element IS5 family transposase	-
ECO25NV_RS25300	133886..135289	+	1404	WP_001373486.1	S-methylmethionine permease	-
ECO25NV_RS25305	135276..136208	+	933	WP_000081352.1	homocysteine S-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B	senB	1..139450	139450
	-	flank	IS/Tn	-	-	125652..126155	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T35355 WP_001216034.1 NZ_AP022327:130879-131259 [Escherichia coli O25:H4]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T35355 NZ_AP022327:130879-131259 [Escherichia coli O25:H4]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT35355 WP_001190712.1 NZ_AP022327:130658-130879 [Escherichia coli O25:H4]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT35355 NZ_AP022327:130658-130879 [Escherichia coli O25:H4]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6