Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4247403..4247979 Replicon chromosome
Accession NZ_AP022326
Organism Escherichia coli O25:H4 strain 18044

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag ECO25NV_RS20810 Protein ID WP_001295743.1
Coordinates 4247692..4247979 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag ECO25NV_RS20805 Protein ID WP_000063148.1
Coordinates 4247403..4247705 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO25NV_RS20790 4243999..4246149 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
ECO25NV_RS20795 4246199..4246402 + 204 WP_000467859.1 YbdD/YjiX family protein -
ECO25NV_RS20800 4246413..4247369 + 957 WP_001295745.1 GTPase -
ECO25NV_RS20805 4247403..4247705 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
ECO25NV_RS20810 4247692..4247979 - 288 WP_001295743.1 BrnT family toxin Toxin
ECO25NV_RS20815 4248176..4249342 + 1167 WP_000800831.1 restriction endonuclease -
ECO25NV_RS20820 4249406..4251025 + 1620 WP_001029745.1 type I restriction-modification system subunit M -
ECO25NV_RS20825 4251015..4252319 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T35341 WP_001295743.1 NZ_AP022326:c4247979-4247692 [Escherichia coli O25:H4]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T35341 NZ_AP022326:c4247979-4247692 [Escherichia coli O25:H4]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT35341 WP_000063148.1 NZ_AP022326:c4247705-4247403 [Escherichia coli O25:H4]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT35341 NZ_AP022326:c4247705-4247403 [Escherichia coli O25:H4]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References