Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4137417..4137937 Replicon chromosome
Accession NZ_AP022326
Organism Escherichia coli O25:H4 strain 18044

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag ECO25NV_RS20280 Protein ID WP_001160964.1
Coordinates 4137417..4137701 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ECO25NV_RS20285 Protein ID WP_000125566.1
Coordinates 4137704..4137937 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO25NV_RS20255 4133062..4134348 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
ECO25NV_RS20260 4134348..4135337 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ECO25NV_RS20265 4135334..4136155 + 822 WP_202270722.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ECO25NV_RS20270 4136158..4136535 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ECO25NV_RS20275 4136542..4137390 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ECO25NV_RS20280 4137417..4137701 - 285 WP_001160964.1 CcdB family protein Toxin
ECO25NV_RS20285 4137704..4137937 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ECO25NV_RS20290 4138023..4138502 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
ECO25NV_RS20295 4138694..4140556 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
ECO25NV_RS20300 4140549..4141079 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ECO25NV_RS20305 4141187..4142518 - 1332 WP_001183209.1 MFS transporter -
ECO25NV_RS20310 4142577..4142864 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T35340 WP_001160964.1 NZ_AP022326:c4137701-4137417 [Escherichia coli O25:H4]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T35340 NZ_AP022326:c4137701-4137417 [Escherichia coli O25:H4]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT35340 WP_000125566.1 NZ_AP022326:c4137937-4137704 [Escherichia coli O25:H4]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT35340 NZ_AP022326:c4137937-4137704 [Escherichia coli O25:H4]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References