Detailed information of TA system TA035320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2064302..2064939
• Replicon: chromosome
• Accession: NZ_AP022322
• Organism: Veillonella sp. S12025-13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KJS32_RS09555
• Protein ID: WP_244856264.1
• Coordinates: 2064538..2064939 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E4LD34
• Locus tag: KJS32_RS09550
• Protein ID: WP_009351573.1
• Coordinates: 2064302..2064532 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJS32_RS09540 (VEIS1202513_18730)	2061787..2062644	+	858	WP_082717874.1	methylenetetrahydrofolate reductase	-
KJS32_RS09545 (VEIS1202513_18740)	2062740..2063492	-	753	WP_213467427.1	16S rRNA (guanine(527)-N(7))-methyltransferase RsmG	-
KJS32_RS09550 (VEIS1202513_18750)	2064302..2064532	+	231	WP_009351573.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KJS32_RS09555 (VEIS1202513_18760)	2064538..2064939	+	402	WP_244856264.1	type II toxin-antitoxin system VapC family toxin	Toxin
KJS32_RS09560 (VEIS1202513_18770)	2065023..2066894	-	1872	WP_213467429.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG	-
KJS32_RS09565 (VEIS1202513_18790)	2067260..2068645	-	1386	WP_213467430.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
KJS32_RS09570 (VEIS1202513_18800)	2068833..2069630	-	798	WP_213467431.1	RNA-binding cell elongation regulator Jag/EloR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-121)

Antitoxin

• SpoVT-AbrB domain (9-44)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14955.30 Da
• Isoelectric Point: 6.6378

>T35320 WP_244856264.1 NZ_AP022322:2064538-2064939 [Veillonella sp. S12025-13]
MLDTNICIYAIKQEPEVVLQKILKHHPSDICISSITYAELMHGVEKSQSKDKNRLALTLLLSPIQIIDFDSHAAEEYGKI
KADLQSQGKVIGPMDLLIASHAKSKDLTIVTNNTKEFKRVNQLEVEDWSKPLS

Nucleotide

• Length: 402 bp

>T35320 NZ_AP022322:2064538-2064939 [Veillonella sp. S12025-13]
ATGTTAGATACTAATATATGTATTTATGCTATCAAACAAGAGCCAGAAGTTGTACTACAGAAAATATTGAAACATCATCC
TTCAGATATATGTATTTCATCAATTACATATGCTGAACTAATGCATGGTGTAGAAAAAAGCCAATCAAAAGATAAAAATA
GATTAGCTTTAACACTTTTATTATCACCTATACAAATTATTGATTTTGACAGCCACGCTGCTGAGGAATATGGAAAAATA
AAAGCCGATTTACAATCACAAGGAAAAGTTATTGGTCCTATGGATCTACTCATTGCAAGCCACGCAAAGTCAAAGGATCT
TACCATTGTTACTAATAATACAAAAGAGTTTAAAAGAGTAAATCAATTAGAAGTTGAAGATTGGTCTAAACCATTAAGCT
AA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8690.84 Da
• Isoelectric Point: 4.4217

>AT35320 WP_009351573.1 NZ_AP022322:2064302-2064532 [Veillonella sp. S12025-13]
MTTAKLFENGRSQAVRLPKEFRFNGDEVIINKIGNVVLLMPKDDEWAGFVAGLSLFTDDFMCNGREDIDHQERETL

Nucleotide

• Length: 231 bp

>AT35320 NZ_AP022322:2064302-2064532 [Veillonella sp. S12025-13]
ATGACCACAGCAAAGCTATTTGAAAATGGCCGTAGCCAGGCTGTACGATTACCAAAGGAATTTCGATTTAATGGTGATGA
AGTAATCATCAACAAAATAGGAAATGTAGTCTTACTTATGCCAAAAGATGATGAATGGGCAGGATTTGTAGCTGGTTTAT
CATTATTTACAGATGATTTTATGTGTAACGGACGGGAAGATATTGATCATCAGGAGCGTGAAACCTTATGA

Structures

Antitoxin

• AlphaFold DB: E4LD34