Detailed information of TA system TA035315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 1180273..1180814
• Replicon: plasmid BTHE68_p1
• Accession: NZ_AP022319
• Organism: Burkholderia sp. THE68

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BTHE68_RS33850
• Protein ID: WP_162070613.1
• Coordinates: 1180512..1180814 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: BTHE68_RS33845
• Protein ID: WP_162070612.1
• Coordinates: 1180273..1180515 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTHE68_RS33820	1175473..1176369	+	897	WP_162070607.1	LysR family transcriptional regulator	-
BTHE68_RS33825	1176465..1177658	+	1194	WP_162070608.1	amidohydrolase	-
BTHE68_RS33830	1177668..1178735	+	1068	WP_162070609.1	M14 family metallopeptidase	-
BTHE68_RS33835	1178935..1179441	+	507	WP_162070610.1	DUF4019 domain-containing protein	-
BTHE68_RS33840	1179449..1179754	-	306	WP_162070611.1	high-potential iron-sulfur protein	-
BTHE68_RS33845	1180273..1180515	+	243	WP_162070612.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BTHE68_RS33850	1180512..1180814	+	303	WP_162070613.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTHE68_RS33855	1180896..1181033	-	138	WP_162070614.1	oxalate:formate antiporter	-
BTHE68_RS33860	1181056..1182453	-	1398	WP_162070615.1	OFA family MFS transporter	-
BTHE68_RS33865	1182974..1184494	+	1521	WP_162070616.1	aldehyde dehydrogenase family protein	-
BTHE68_RS33870	1184551..1184940	+	390	WP_162070617.1	DUF779 domain-containing protein	-
BTHE68_RS33875	1184946..1185725	-	780	WP_162070618.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	tssC-5 / pvdF	1..1527330	1527330

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-91)

Antitoxin

• Antitoxin ParD (4-72)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11317.95 Da
• Isoelectric Point: 7.2764

>T35315 WP_162070613.1 NZ_AP022319:1180512-1180814 [Burkholderia sp. THE68]
MKSCLFTLAAIQDLDGIWDYTFDKWGPAQAERYARMIQDTVSGLGKGAQRSLSAEHIRTGYRKALVGMHVIFFRESEDTI
QFVRILRQQMDLPAHLGDAG

Nucleotide

• Length: 303 bp

>T35315 NZ_AP022319:1180512-1180814 [Burkholderia sp. THE68]
ATGAAGTCATGCCTGTTTACGCTGGCGGCGATCCAGGATCTCGACGGCATTTGGGATTACACGTTCGACAAGTGGGGTCC
GGCTCAGGCCGAGCGGTATGCGCGAATGATTCAGGACACGGTCAGTGGCCTCGGCAAGGGCGCCCAGCGGAGTCTGTCGG
CGGAACACATTCGCACCGGCTACCGTAAAGCCCTGGTCGGAATGCACGTGATCTTCTTCAGGGAATCCGAGGATACGATC
CAGTTCGTGCGGATACTCCGTCAACAGATGGATTTGCCGGCCCATCTAGGCGACGCCGGATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8895.98 Da
• Isoelectric Point: 10.4416

>AT35315 WP_162070612.1 NZ_AP022319:1180273-1180515 [Burkholderia sp. THE68]
MAKNTSVSLGDHFADFVENRVRAGRYSTASDVVRAGLRLLEEQEARLDTLRSALEAGERPGSSKPLDFDAFIARKRKSTR

Nucleotide

• Length: 243 bp

>AT35315 NZ_AP022319:1180273-1180515 [Burkholderia sp. THE68]
ATGGCCAAAAATACGTCCGTTTCGCTCGGCGATCATTTCGCGGATTTCGTCGAAAATCGCGTTCGCGCTGGCCGCTACAG
CACTGCAAGCGATGTGGTCCGTGCCGGATTGCGCCTGCTCGAAGAACAGGAAGCGAGGCTCGACACACTCCGTTCCGCCC
TGGAAGCGGGAGAGCGTCCGGGGTCGTCGAAGCCGCTCGATTTCGATGCGTTCATCGCCCGTAAGCGCAAGTCCACTCGA
TGA