Detailed information of TA system TA035308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 827537..828221
• Replicon: chromosome
• Accession: NZ_AP022315
• Organism: Burkholderia sp. THE68

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: BTHE68_RS03950
• Protein ID: WP_162065228.1
• Coordinates: 827537..827905 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: BTHE68_RS03955
• Protein ID: WP_162066775.1
• Coordinates: 827898..828221 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTHE68_RS03940	822999..824978	+	1980	WP_162065226.1	type IIA DNA topoisomerase subunit B	-
BTHE68_RS03945	825017..827335	+	2319	WP_162065227.1	DNA topoisomerase IV subunit A	-
BTHE68_RS03950	827537..827905	+	369	WP_162065228.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTHE68_RS03955	827898..828221	+	324	WP_162066775.1	helix-turn-helix domain-containing protein	Antitoxin
BTHE68_RS03960	828228..828494	-	267	WP_162065229.1	hypothetical protein	-
BTHE68_RS03965	828733..829191	+	459	WP_162065230.1	CopD family protein	-
BTHE68_RS03970	829212..831641	-	2430	WP_162065231.1	EAL domain-containing protein	-
BTHE68_RS03975	832036..832926	-	891	WP_162065232.1	PhzF family phenazine biosynthesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-110)

Antitoxin

• HigA2-like, helix-turn-helix domain (11-89)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13575.54 Da
• Isoelectric Point: 10.0648

>T35308 WP_162065228.1 NZ_AP022315:827537-827905 [Burkholderia sp. THE68]
MEEREVKPLRWVGSAKRDLKTMPADVQDMFGFALYQAQSGRKHEQAKPLKGFGSAGVLEVVESSKDGAFRAVYTVRFEHA
VYVLHCFQKKSASGIATPRPDMEIIRARLKAAQAHAAGERND

Nucleotide

• Length: 369 bp

>T35308 NZ_AP022315:827537-827905 [Burkholderia sp. THE68]
ATGGAAGAGCGAGAAGTCAAGCCGCTGCGATGGGTGGGTTCCGCGAAGCGAGACCTGAAGACAATGCCAGCGGACGTGCA
GGACATGTTTGGCTTTGCGCTGTATCAGGCGCAGTCCGGCAGAAAGCACGAACAGGCGAAGCCGTTGAAGGGATTCGGAT
CTGCAGGCGTGCTGGAAGTCGTGGAATCGTCGAAGGATGGTGCTTTTCGCGCGGTCTATACGGTGAGATTCGAGCACGCG
GTTTATGTGTTGCACTGTTTCCAGAAGAAATCGGCATCTGGCATCGCCACACCGAGACCGGATATGGAGATCATCCGGGC
GCGGCTGAAAGCGGCCCAGGCCCATGCAGCGGGAGAGCGCAATGATTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11751.69 Da
• Isoelectric Point: 10.7886

>AT35308 WP_162066775.1 NZ_AP022315:827898-828221 [Burkholderia sp. THE68]
MIEIEDSSGNVYADLGLPDADEMRVKAQLAGKIGEIIKTRRWTQQRAADVLGISQPKLSQLLRGQFRGISEAKMLELLAR
LGRNVQIVVGPPRRSAKPGRVEVVFAA

Nucleotide

• Length: 324 bp

>AT35308 NZ_AP022315:827898-828221 [Burkholderia sp. THE68]
ATGATTGAGATAGAAGATAGCAGCGGCAACGTGTACGCTGACCTGGGACTGCCCGATGCCGACGAAATGCGCGTCAAGGC
GCAACTCGCCGGCAAGATTGGCGAAATCATCAAGACCCGGCGCTGGACGCAGCAACGTGCCGCTGATGTTCTCGGCATCA
GCCAACCCAAGCTCTCGCAACTGCTGCGCGGCCAGTTTCGGGGCATCAGCGAAGCGAAGATGCTCGAGTTGCTCGCGCGC
CTTGGCCGCAATGTGCAAATCGTCGTCGGTCCGCCGCGGCGCTCGGCCAAGCCAGGCCGCGTGGAAGTCGTGTTCGCCGC
CTAG