Detailed information of TA system TA035306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 657257..657891
• Replicon: chromosome
• Accession: NZ_AP022315
• Organism: Burkholderia sp. THE68

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BTHE68_RS03110
• Protein ID: WP_162065096.1
• Coordinates: 657257..657436 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BTHE68_RS03115
• Protein ID: WP_162065097.1
• Coordinates: 657472..657891 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTHE68_RS03105	654901..657030	-	2130	WP_174257982.1	methionine--tRNA ligase	-
BTHE68_RS03110	657257..657436	+	180	WP_162065096.1	type II toxin-antitoxin system HicA family toxin	Toxin
BTHE68_RS03115	657472..657891	+	420	WP_162065097.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BTHE68_RS03120	658132..658788	-	657	WP_061173047.1	OmpA family protein	-
BTHE68_RS03125	659052..660140	+	1089	WP_061173113.1	iron-sulfur cluster carrier protein ApbC	-
BTHE68_RS03130	660260..660793	+	534	WP_162065098.1	superoxide dismutase family protein	-
BTHE68_RS03135	660882..661451	+	570	WP_035508313.1	dCTP deaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-70)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6714.93 Da
• Isoelectric Point: 11.5470

>T35306 WP_162065096.1 NZ_AP022315:657257-657436 [Burkholderia sp. THE68]
MKYSEFRKWLRKQGGIFTPAKGSHFRVTLNGKATIFPDHGSKEIGTNLVVRIKKQLGLK

Nucleotide

• Length: 180 bp

>T35306 NZ_AP022315:657257-657436 [Burkholderia sp. THE68]
ATGAAATACAGTGAGTTCAGGAAGTGGTTGCGCAAGCAGGGCGGGATTTTTACTCCTGCGAAAGGCAGCCATTTTCGCGT
GACACTCAACGGCAAGGCCACCATCTTTCCTGATCACGGCAGCAAGGAGATCGGAACGAATCTGGTGGTGCGAATCAAGA
AACAGCTTGGGCTGAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15508.00 Da
• Isoelectric Point: 5.2666

>AT35306 WP_162065097.1 NZ_AP022315:657472-657891 [Burkholderia sp. THE68]
MLSYPVNLELDTNNTFLVTFPDIPEAFSVGDDEGEALINGLDALESAIEIYFDEKREVPMPSKPKKGQPVVTLPALVVTK
VLLQNEMLRQGVRKSELARRLNVHMPQVDRLLDPRHSSKLEAIEAAFLSLGKRLKISLA

Nucleotide

• Length: 420 bp

>AT35306 NZ_AP022315:657472-657891 [Burkholderia sp. THE68]
ATGCTGTCCTATCCAGTGAATCTGGAACTCGACACAAACAACACTTTTCTGGTCACGTTTCCCGACATACCGGAAGCATT
TTCCGTAGGCGATGATGAAGGTGAGGCGCTAATCAACGGACTCGACGCACTGGAAAGCGCTATCGAAATTTACTTCGACG
AAAAACGCGAAGTGCCTATGCCGTCGAAACCGAAGAAAGGTCAACCGGTCGTCACTTTGCCCGCGCTCGTCGTTACAAAG
GTGCTGCTTCAGAACGAAATGCTTCGTCAGGGCGTGCGCAAGTCCGAACTCGCGCGGCGACTCAACGTTCACATGCCGCA
AGTCGACCGCCTGCTCGACCCGCGACATTCGTCGAAACTCGAAGCGATCGAAGCCGCGTTTCTGTCTCTGGGCAAGCGCC
TGAAAATTTCGTTGGCGTGA