Detailed information of TA system TA035303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 4147538..4148082
• Replicon: chromosome
• Accession: NZ_AP022314
• Organism: Mycobacterium xenopi strain JCM 15661

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I0RYT9
• Locus tag: MYXE_RS19775
• Protein ID: WP_003919576.1
• Coordinates: 4147538..4147864 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MYXE_RS19780
• Protein ID: WP_085195272.1
• Coordinates: 4147861..4148082 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYXE_RS19745	4143724..4144518	-	795	WP_085195281.1	hypothetical protein	-
MYXE_RS19750	4144703..4144882	-	180	WP_085195279.1	hypothetical protein	-
MYXE_RS19755	4144882..4145316	-	435	WP_083088679.1	type II toxin-antitoxin system VapC family toxin	-
MYXE_RS19760	4145313..4145609	-	297	WP_085195277.1	toxin-antitoxin system HicB family antitoxin	-
MYXE_RS19765	4145919..4146302	-	384	WP_085195307.1	nuclear transport factor 2 family protein	-
MYXE_RS19770	4146552..4146821	-	270	WP_085195275.1	hypothetical protein	-
MYXE_RS19775	4147538..4147864	-	327	WP_003919576.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MYXE_RS19780	4147861..4148082	-	222	WP_085195272.1	antitoxin MazE family protein	Antitoxin
MYXE_RS19785	4148247..4148528	-	282	WP_085195270.1	type II toxin-antitoxin system PemK/MazF family toxin	-
MYXE_RS19790	4148515..4148760	-	246	WP_085195305.1	ribbon-helix-helix protein, CopG family	-
MYXE_RS19795	4148990..4149325	-	336	WP_085195268.1	DUF86 domain-containing protein	-
MYXE_RS19800	4149315..4149620	-	306	WP_085195266.1	nucleotidyltransferase domain-containing protein	-
MYXE_RS19805	4149859..4152171	-	2313	WP_085195264.1	GH92 family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (7-69)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11625.49 Da
• Isoelectric Point: 6.9795

>T35303 WP_003919576.1 NZ_AP022314:c4147864-4147538 [Mycobacterium xenopi]
VRRGELWTAAGGRHYAGKPRPVVIVQDDRFDATSSITVCPLTSDPTEIPLLRVPLSPSELNGLAAPSSIMLDKVTTMPRS
KLGELIGNVSDAEMLEVSRRLVVFLGLA

Nucleotide

• Length: 327 bp

>T35303 NZ_AP022314:c4147864-4147538 [Mycobacterium xenopi]
GTGAGACGCGGAGAGCTCTGGACGGCTGCTGGCGGCAGGCATTACGCCGGCAAACCGCGCCCGGTGGTCATAGTCCAGGA
CGATCGGTTTGATGCGACCTCGTCGATTACCGTCTGTCCGCTGACGAGCGATCCCACCGAGATTCCGCTGCTGCGAGTCC
CGCTTTCGCCGAGCGAACTCAACGGGCTGGCTGCGCCGAGCAGCATCATGCTCGACAAGGTGACCACGATGCCCCGGTCC
AAGCTAGGTGAGCTCATCGGGAACGTCTCTGACGCAGAGATGCTAGAGGTCTCCAGGCGGCTCGTGGTTTTTCTAGGCCT
GGCTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8254.02 Da
• Isoelectric Point: 4.4678

>AT35303 WP_085195272.1 NZ_AP022314:c4148082-4147861 [Mycobacterium xenopi]
VPEREVRQRVADYRERLRRQGLRPVQIWVPDVRAPGFAAEAHRQSALAAASPYEGDDQAFVDAISSFDDGEEE

Nucleotide

• Length: 222 bp

>AT35303 NZ_AP022314:c4148082-4147861 [Mycobacterium xenopi]
GTGCCTGAGCGTGAAGTTCGTCAACGGGTCGCTGACTATCGGGAACGTTTGCGCCGGCAGGGTTTGCGCCCGGTACAGAT
CTGGGTTCCCGATGTCCGAGCGCCAGGATTCGCGGCTGAGGCTCACCGTCAGTCGGCATTGGCGGCAGCCAGCCCTTATG
AGGGTGATGATCAGGCATTCGTCGACGCGATCTCGTCGTTTGATGACGGCGAGGAGGAGTGA

Structures

Toxin

• AlphaFold DB: I0RYT9