Detailed information of TA system TA035301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2489000..2489586
• Replicon: chromosome
• Accession: NZ_AP022314
• Organism: Mycobacterium xenopi strain JCM 15661

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I0RF21
• Locus tag: MYXE_RS11565
• Protein ID: WP_003922982.1
• Coordinates: 2489242..2489586 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MYXE_RS11560
• Protein ID: WP_039891297.1
• Coordinates: 2489000..2489248 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYXE_RS11535	2484567..2485076	+	510	WP_085194013.1	hypothetical protein	-
MYXE_RS11540	2485146..2485898	-	753	WP_112650037.1	SWF/SNF helicase family protein	-
MYXE_RS11545	2485794..2486387	+	594	WP_003920154.1	hypothetical protein	-
MYXE_RS11550	2486502..2487960	+	1459	Protein_2296	DDE-type integrase/transposase/recombinase	-
MYXE_RS11555	2487957..2488773	+	817	Protein_2297	ExeA family protein	-
MYXE_RS11560	2489000..2489248	+	249	WP_039891297.1	ribbon-helix-helix protein, CopG family	Antitoxin
MYXE_RS11565	2489242..2489586	+	345	WP_003922982.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MYXE_RS11570	2489854..2490212	+	359	Protein_2300	nuclear transport factor 2 family protein	-
MYXE_RS24015	2490226..2490366	-	141	WP_197904862.1	hypothetical protein	-
MYXE_RS11580	2490563..2490781	-	219	WP_085194514.1	HNH endonuclease	-
MYXE_RS11585	2490968..2491246	-	279	WP_085194516.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MYXE_RS11590	2491243..2491512	-	270	WP_085194519.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MYXE_RS23605	2491607..2491960	+	354	WP_085194521.1	hypothetical protein	-
MYXE_RS11595	2492251..2492679	-	429	WP_003918897.1	cellulose-binding domain-containing protein	-
MYXE_RS11600	2492964..2493950	+	987	WP_039889418.1	peroxiredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12306.20 Da
• Isoelectric Point: 10.4059

>T35301 WP_003922982.1 NZ_AP022314:2489242-2489586 [Mycobacterium xenopi]
MVISRGEIYWADLGPPSGSRPAKRRPVLVIQSDPYNASRLATTLAAVITSNTALATMPGNVFLPATTTRLPRDSVVNATA
LVTLNKTDLTDRVGEVPPSLMHEVDRGLRRVLDL

Nucleotide

• Length: 345 bp

>T35301 NZ_AP022314:2489242-2489586 [Mycobacterium xenopi]
ATGGTGATCAGCCGCGGTGAGATTTACTGGGCGGACCTCGGACCGCCGTCCGGCAGCCGGCCCGCCAAGCGCCGTCCGGT
TCTAGTAATCCAATCGGATCCGTATAACGCCAGTCGCCTTGCTACCACGCTCGCGGCAGTGATCACGTCCAACACGGCAT
TAGCGACGATGCCGGGCAATGTTTTCTTGCCCGCGACCACAACGAGACTGCCACGTGATTCGGTCGTCAACGCCACGGCG
CTCGTTACGCTGAACAAAACCGACCTCACCGACCGAGTCGGAGAGGTGCCACCCAGCCTGATGCATGAAGTCGATAGAGG
ACTTCGCCGCGTGCTTGACCTTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9155.02 Da
• Isoelectric Point: 4.1096

>AT35301 WP_039891297.1 NZ_AP022314:2489000-2489248 [Mycobacterium xenopi]
VKTAISLPDETFDRVSRRASELGMSRSEFFTRAAQRYLDELDAQSLTGQIDMALENLHDTDEAEAAAVTVGHRVLDAVDD
EW

Nucleotide

• Length: 249 bp

>AT35301 NZ_AP022314:2489000-2489248 [Mycobacterium xenopi]
GTGAAGACAGCCATTTCTCTCCCCGACGAGACGTTCGATCGGGTTTCCCGGCGAGCGAGCGAGCTAGGCATGAGCCGGTC
AGAGTTCTTCACCCGTGCTGCCCAGCGCTACCTGGACGAATTAGACGCCCAATCGCTGACCGGCCAGATCGACATGGCTC
TGGAGAACCTTCATGACACCGACGAAGCGGAGGCCGCCGCTGTGACCGTCGGGCATCGCGTGTTAGACGCAGTGGATGAT
GAATGGTGA

Structures

Toxin

• AlphaFold DB: I0RF21