Detailed information of TA system TA035300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1014679..1015310
• Replicon: chromosome
• Accession: NZ_AP022314
• Organism: Mycobacterium xenopi strain JCM 15661

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: I0RFG9
• Locus tag: MYXE_RS04625
• Protein ID: WP_003922521.1
• Coordinates: 1014679..1015056 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I0RFH0
• Locus tag: MYXE_RS04630
• Protein ID: WP_003922522.1
• Coordinates: 1015053..1015310 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYXE_RS04600	1009805..1010977	-	1173	WP_085197062.1	cytochrome P450	-
MYXE_RS04605	1011221..1011653	+	433	Protein_917	helix-turn-helix transcriptional regulator	-
MYXE_RS04610	1011658..1012707	-	1050	WP_161552045.1	spirocyclase AveC family protein	-
MYXE_RS04615	1013393..1013944	-	552	WP_157139298.1	RES domain-containing protein	-
MYXE_RS04620	1013941..1014273	-	333	WP_003922519.1	DUF2384 domain-containing protein	-
MYXE_RS04625	1014679..1015056	-	378	WP_003922521.1	type II toxin-antitoxin system VapC family toxin	Toxin
MYXE_RS04630	1015053..1015310	-	258	WP_003922522.1	ribbon-helix-helix domain-containing protein	Antitoxin
MYXE_RS04635	1015416..1016009	-	594	WP_085197055.1	thioredoxin family protein	-
MYXE_RS04640	1016154..1016846	+	693	WP_085197053.1	D-alanyl-D-alanine dipeptidase	-
MYXE_RS04645	1016923..1017337	+	415	Protein_925	SAM-dependent methyltransferase	-
MYXE_RS04650	1017365..1018903	-	1539	WP_039891118.1	amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13650.67 Da
• Isoelectric Point: 4.9440

>T35300 WP_003922521.1 NZ_AP022314:c1015056-1014679 [Mycobacterium xenopi]
VKLIDTTIAVDHLRGEPAAAELLAALTATGEELVASELVRFELLAGVRDKELGAFEEFCSALRWAAVTEDVARVGGRLAR
RYRRSHSGIDAVDYLIAATAIVIDADLLTTNVRHFPMFAELKPPY

Nucleotide

• Length: 378 bp

>T35300 NZ_AP022314:c1015056-1014679 [Mycobacterium xenopi]
GTGAAGCTGATTGACACCACCATCGCTGTCGACCATTTGCGGGGCGAGCCCGCGGCTGCCGAGCTGCTCGCGGCACTGAC
CGCCACCGGTGAGGAATTGGTGGCAAGTGAACTGGTCCGGTTCGAGCTCCTGGCGGGCGTACGGGACAAGGAACTCGGCG
CGTTTGAGGAGTTCTGCTCCGCCCTGCGTTGGGCCGCCGTGACCGAGGATGTGGCACGCGTCGGTGGACGACTCGCGCGT
CGGTACCGAAGAAGCCATAGCGGCATCGACGCTGTCGACTACCTGATCGCCGCGACCGCGATCGTCATCGACGCCGACCT
GCTGACGACGAATGTGCGCCACTTCCCGATGTTTGCCGAGCTGAAGCCCCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9454.57 Da
• Isoelectric Point: 10.6506

>AT35300 WP_003922522.1 NZ_AP022314:c1015310-1015053 [Mycobacterium xenopi]
MRTQITLNDEEVELLDRAVKATGASRSELIRRAIRATYGSRSKEERVAALKRSAGSWRGRDFTGADYVDAVRGDLNERLS
RLGSA

Nucleotide

• Length: 258 bp

>AT35300 NZ_AP022314:c1015310-1015053 [Mycobacterium xenopi]
ATGCGCACCCAGATCACGCTTAATGATGAGGAGGTCGAGCTCCTCGATCGCGCTGTCAAGGCAACTGGCGCATCCCGGTC
CGAGCTGATCCGTCGGGCGATTCGCGCTACTTACGGAAGCCGAAGCAAGGAAGAGCGGGTGGCGGCCCTGAAGCGAAGCG
CCGGCTCCTGGCGGGGACGTGACTTCACCGGTGCTGACTACGTGGACGCTGTTCGCGGTGACCTCAACGAGCGCCTTAGC
CGGCTCGGTTCGGCGTGA

Structures

Toxin

• AlphaFold DB: I0RFG9

Antitoxin

• AlphaFold DB: I0RFH0