Detailed information of TA system TA035299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4735..5320
• Replicon: plasmid pBEC1-S18-ESBL-01_2
• Accession: NZ_AP022304
• Organism: Acinetobacter sp. BEC1-S18-ESBL-01

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A158LPL9
• Locus tag: H7R46_RS19165
• Protein ID: WP_002124950.1
• Coordinates: 4735..5022 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: H7R46_RS19170
• Protein ID: WP_086260804.1
• Coordinates: 5009..5320 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R46_RS19145	101..1030	+	930	WP_005065049.1	replication initiation protein RepM	-
H7R46_RS19150	1109..1639	+	531	WP_005065050.1	plasmid replication DNA-binding protein	-
H7R46_RS19155	1871..2722	-	852	WP_005065068.1	universal stress protein	-
H7R46_RS19160	2735..4222	-	1488	WP_005065066.1	SulP family inorganic anion transporter	-
H7R46_RS19165	4735..5022	+	288	WP_002124950.1	BrnT family toxin	Toxin
H7R46_RS19170	5009..5320	+	312	WP_086260804.1	BrnA antitoxin family protein	Antitoxin
H7R46_RS19175	5411..6085	-	675	WP_005065062.1	HEPN domain-containing protein	-
H7R46_RS19180	6137..6538	-	402	WP_005065059.1	hypothetical protein	-
H7R46_RS19185	6630..6932	-	303	WP_005065057.1	XRE family transcriptional regulator	-
H7R46_RS19190	6937..7281	-	345	WP_005065055.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H7R46_RS19195	7802..8119	-	318	WP_000900860.1	hypothetical protein	-
H7R46_RS19200	8508..9392	-	885	WP_005065070.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11158	11158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11173.58 Da
• Isoelectric Point: 4.9093

>T35299 WP_002124950.1 NZ_AP022304:4735-5022 [Acinetobacter sp. BEC1-S18-ESBL-01]
MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNGEERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T35299 NZ_AP022304:4735-5022 [Acinetobacter sp. BEC1-S18-ESBL-01]
ATGGAACTGTATTTCGAATGGGATGAGGCTAAAAACCAAAAGAATCAGAAAAAACATGATGTATCTTTTGAAACGGCAAG
CCTCGTTTTTGAAGATCCTTTACGGATCTCAATCCAGGATCGACATACCAATGGTGAAGAACGTTGGCAAACGATCGGTA
TGGTTAAAGGTGTACTGATGTTATTGGTGGCTCATACAATCATTGACGATGATGACTGTGAGATCATACGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAACGCAATCAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 12070.80 Da
• Isoelectric Point: 9.4651

>AT35299 WP_086260804.1 NZ_AP022304:5009-5320 [Acinetobacter sp. BEC1-S18-ESBL-01]
MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEITDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQ
GKGYQTRMNKILREAMLEDIKNH

Nucleotide

• Length: 312 bp

>AT35299 NZ_AP022304:5009-5320 [Acinetobacter sp. BEC1-S18-ESBL-01]
ATGAGCATGGTTAGATATACACGCAAAGAACTGAATGAAAAATTCAGTGACAAGCAAGATGCTGAAATTAAACGCTTGCT
TGCAAAAGGAACTGTACCAGATGAACAATTAGATCTATCTGACATTCCTGAGATTACCGACTGGAGTCATGCTGTTCGCC
ATGGGCAATTTTATCGTCCAGTGAAGCAACAAACATCTGTTCGTCTAGATGCAGATGTACTTGCATGGTTAAAGGCTCAA
GGCAAAGGCTATCAGACACGCATGAATAAAATTCTGCGTGAAGCAATGCTAGAAGATATTAAGAATCATTGA

Structures

Toxin

• AlphaFold DB: A0A158LPL9