Detailed information of TA system TA035291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 101442..102097
• Replicon: plasmid pBEC1-S17-ESBL-09_1
• Accession: NZ_AP022299
• Organism: Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A2B7QJJ1
• Locus tag: H7R48_RS25040
• Protein ID: WP_001441071.1
• Coordinates: 101696..102097 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A0E0YAG4
• Locus tag: H7R48_RS25035
• Protein ID: WP_001261288.1
• Coordinates: 101442..101672 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R48_RS25000	96859..97251	-	393	WP_000340832.1	plasmid partitioning/stability family protein	-
H7R48_RS25005	97256..98227	-	972	WP_001103689.1	hypothetical protein	-
H7R48_RS25010	98455..99099	+	645	WP_000633911.1	AAA family ATPase	-
H7R48_RS25015	99093..99368	+	276	WP_000239529.1	hypothetical protein	-
H7R48_RS25020	99513..100322	-	810	WP_000016979.1	site-specific integrase	-
H7R48_RS25025	100323..100628	-	306	WP_071701705.1	type II toxin-antitoxin system toxin CcdB	-
H7R48_RS25030	100630..100848	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
H7R48_RS25035	101442..101672	+	231	WP_001261288.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H7R48_RS25040	101696..102097	+	402	WP_001441071.1	type II toxin-antitoxin system VapC family toxin	Toxin
H7R48_RS25045	102521..103675	-	1155	Protein_119	IS4-like element IS4 family transposase	-
H7R48_RS25050	103726..104195	-	470	Protein_120	protein ImpB	-
H7R48_RS25055	104195..104632	-	438	WP_139451311.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
H7R48_RS25060	104629..104877	-	249	WP_000618110.1	DinI-like family protein	-
H7R48_RS25065	105152..106054	+	903	WP_139451382.1	DUF1281 domain-containing protein	-
H7R48_RS25070	106126..106788	-	663	Protein_124	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / agg3D / agg3C / agg3B / agg3A / aap/aspU	1..123266	123266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14517.89 Da
• Isoelectric Point: 7.2456

>T35291 WP_001441071.1 NZ_AP022299:101696-102097 [Escherichia coli]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFGATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 402 bp

>T35291 NZ_AP022299:101696-102097 [Escherichia coli]
ATGCTCGATACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGGCGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGGGACGCCAATCGGCCCGAACGACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8684.76 Da
• Isoelectric Point: 4.3654

>AT35291 WP_001261288.1 NZ_AP022299:101442-101672 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT35291 NZ_AP022299:101442-101672 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A2B7QJJ1

Antitoxin

• AlphaFold DB: A0A0E0YAG4