Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 100323..100848 Replicon plasmid pBEC1-S17-ESBL-09_1
Accession NZ_AP022299
Organism Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)


Gene name ccdB Uniprot ID A0A853WMN3
Locus tag H7R48_RS25025 Protein ID WP_071701705.1
Coordinates 100323..100628 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H7R48_RS25030 Protein ID WP_000813634.1
Coordinates 100630..100848 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R48_RS24990 95498..95923 - 426 WP_182924667.1 hypothetical protein -
H7R48_RS24995 96030..96827 + 798 Protein_109 translesion error-prone DNA polymerase V subunit UmuC -
H7R48_RS25000 96859..97251 - 393 WP_000340832.1 plasmid partitioning/stability family protein -
H7R48_RS25005 97256..98227 - 972 WP_001103689.1 hypothetical protein -
H7R48_RS25010 98455..99099 + 645 WP_000633911.1 AAA family ATPase -
H7R48_RS25015 99093..99368 + 276 WP_000239529.1 hypothetical protein -
H7R48_RS25020 99513..100322 - 810 WP_000016979.1 site-specific integrase -
H7R48_RS25025 100323..100628 - 306 WP_071701705.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R48_RS25030 100630..100848 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R48_RS25035 101442..101672 + 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R48_RS25040 101696..102097 + 402 WP_001441071.1 type II toxin-antitoxin system VapC family toxin -
H7R48_RS25045 102521..103675 - 1155 Protein_119 IS4-like element IS4 family transposase -
H7R48_RS25050 103726..104195 - 470 Protein_120 protein ImpB -
H7R48_RS25055 104195..104632 - 438 WP_139451311.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
H7R48_RS25060 104629..104877 - 249 WP_000618110.1 DinI-like family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pic / agg3D / agg3C / agg3B / agg3A / aap/aspU 1..123266 123266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T35290 WP_071701705.1 NZ_AP022299:c100628-100323 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLATARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T35290 NZ_AP022299:c100628-100323 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCACTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT35290 WP_000813634.1 NZ_AP022299:c100848-100630 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT35290 NZ_AP022299:c100848-100630 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGCCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853WMN3

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References