Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 89404..89963 Replicon plasmid pBEC1-S17-ESBL-09_1
Accession NZ_AP022299
Organism Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)


Gene name relE Uniprot ID G4VUF8
Locus tag H7R48_RS24945 Protein ID WP_000421273.1
Coordinates 89688..89963 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag H7R48_RS24940 Protein ID WP_001387467.1
Coordinates 89404..89688 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R48_RS24915 84789..85355 - 567 Protein_93 zinc-binding dehydrogenase -
H7R48_RS24920 85708..86598 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
H7R48_RS24925 86623..87003 + 381 WP_001403366.1 Rid family detoxifying hydrolase -
H7R48_RS24930 87036..88001 + 966 WP_023063778.1 2-keto-3-deoxygluconate transporter -
H7R48_RS24935 88047..88799 - 753 WP_089627897.1 IclR family transcriptional regulator -
H7R48_RS24940 89404..89688 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
H7R48_RS24945 89688..89963 + 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R48_RS24950 90069..90362 + 294 WP_001105065.1 hypothetical protein -
H7R48_RS24955 90397..90525 - 129 Protein_101 IS481 family transposase -
H7R48_RS24960 90558..91727 - 1170 WP_139451294.1 hypothetical protein -
H7R48_RS24965 92220..92527 + 308 Protein_103 type II toxin-antitoxin system ParD family antitoxin -
H7R48_RS24970 92643..92804 + 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
H7R48_RS24975 92871..93053 + 183 WP_170986216.1 serine/threonine protein kinase -
H7R48_RS24980 93174..93914 + 741 WP_001584328.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pic / agg3D / agg3C / agg3B / agg3A / aap/aspU 1..123266 123266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10765.45 Da        Isoelectric Point: 9.9324

>T35289 WP_000421273.1 NZ_AP022299:89688-89963 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T35289 NZ_AP022299:89688-89963 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT35289 WP_001387467.1 NZ_AP022299:89404-89688 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT35289 NZ_AP022299:89404-89688 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6H1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References