Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4634930..4635427 Replicon chromosome
Accession NZ_AP022298
Organism Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H7R48_RS22265 Protein ID WP_001173343.1
Coordinates 4634930..4635103 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H7R48_RS22270 Protein ID WP_000398619.1
Coordinates 4635131..4635427 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R48_RS22255 4631601..4633058 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
H7R48_RS22260 4633295..4634812 + 1518 WP_000003806.1 lysine--tRNA ligase -
H7R48_RS22265 4634930..4635103 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H7R48_RS22270 4635131..4635427 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H7R48_RS22275 4635656..4635928 - 273 WP_000405647.1 N-acetyltransferase -
H7R48_RS22280 4635940..4636170 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
H7R48_RS22285 4636351..4637982 + 1632 WP_001216477.1 sensor histidine kinase -
H7R48_RS22290 4637979..4638698 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - iroB 4575761..4635427 59666


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T35280 WP_001173343.1 NZ_AP022298:c4635103-4634930 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T35280 NZ_AP022298:c4635103-4634930 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT35280 WP_000398619.1 NZ_AP022298:c4635427-4635131 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT35280 NZ_AP022298:c4635427-4635131 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References