Detailed information of TA system TA035278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4442285..4442880
• Replicon: chromosome
• Accession: NZ_AP022298
• Organism: Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: H7R48_RS21195
• Protein ID: WP_000239581.1
• Coordinates: 4442285..4442635 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: H7R48_RS21200
• Protein ID: WP_001223213.1
• Coordinates: 4442629..4442880 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R48_RS21175	4437732..4438754	-	1023	WP_001305656.1	ABC transporter permease	-
H7R48_RS21180	4438768..4440270	-	1503	WP_000205837.1	sugar ABC transporter ATP-binding protein	-
H7R48_RS21185	4440410..4441366	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
H7R48_RS21190	4441676..4442206	+	531	WP_000055075.1	inorganic diphosphatase	-
H7R48_RS21195	4442285..4442635	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
H7R48_RS21200	4442629..4442880	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
H7R48_RS21205	4443171..4444166	-	996	WP_001519231.1	tyrosine-type recombinase/integrase	-
H7R48_RS21210	4444233..4444532	-	300	WP_000108576.1	helix-turn-helix transcriptional regulator	-
H7R48_RS21215	4444641..4445066	+	426	WP_001158283.1	regulatory protein	-
H7R48_RS21220	4445104..4445454	+	351	WP_044860303.1	DUF4761 family protein	-
H7R48_RS21225	4445465..4445752	+	288	WP_044860302.1	hypothetical protein	-
H7R48_RS21230	4445764..4446006	+	243	WP_000514277.1	DUF4754 family protein	-
H7R48_RS21235	4446003..4446116	+	114	WP_044860301.1	hypothetical protein	-
H7R48_RS21240	4446210..4446620	+	411	WP_044860300.1	hypothetical protein	-
H7R48_RS21245	4446667..4446846	+	180	WP_044860307.1	hypothetical protein	-
H7R48_RS21250	4446843..4447088	+	246	WP_032082656.1	winged helix-turn-helix domain-containing protein	-
H7R48_RS21255	4447085..4447384	+	300	WP_044860299.1	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4436750..4481096	44346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T35278 WP_000239581.1 NZ_AP022298:c4442635-4442285 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T35278 NZ_AP022298:c4442635-4442285 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT35278 WP_001223213.1 NZ_AP022298:c4442880-4442629 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT35278 NZ_AP022298:c4442880-4442629 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATTTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7