Detailed information of TA system TA035274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4181380..4181819
• Replicon: chromosome
• Accession: NZ_AP022298
• Organism: Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H7R48_RS19920
• Protein ID: WP_088539633.1
• Coordinates: 4181380..4181583 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1NMQ0
• Locus tag: H7R48_RS19925
• Protein ID: WP_000125568.1
• Coordinates: 4181586..4181819 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R48_RS19890	4176942..4178228	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
H7R48_RS19895	4178228..4179217	+	990	WP_088539631.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
H7R48_RS19900	4179214..4180035	+	822	WP_075861932.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
H7R48_RS19905	4180038..4180415	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
H7R48_RS19910	4180422..4181270	+	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
H7R48_RS19915	4181267..4181383	-	117	WP_000148634.1	CcdB family protein	-
H7R48_RS19920	4181380..4181583	-	204	WP_088539633.1	CcdB family protein	Toxin
H7R48_RS19925	4181586..4181819	-	234	WP_000125568.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H7R48_RS19930	4181905..4182384	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
H7R48_RS19935	4182576..4184438	-	1863	WP_000377112.1	glutathione-regulated potassium-efflux system protein KefC	-
H7R48_RS19940	4184431..4184961	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
H7R48_RS19945	4185069..4186400	-	1332	WP_001183201.1	MFS transporter	-
H7R48_RS19950	4186458..4186745	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-66)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 7784.15 Da
• Isoelectric Point: 10.5893

>T35274 WP_088539633.1 NZ_AP022298:c4181583-4181380 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLS

Nucleotide

• Length: 204 bp

>T35274 NZ_AP022298:c4181583-4181380 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGATAGATGGCAAAGAATATGTGCTCTCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.55 Da
• Isoelectric Point: 5.8062

>AT35274 WP_000125568.1 NZ_AP022298:c4181819-4181586 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT35274 NZ_AP022298:c4181819-4181586 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A829GEF2