Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3745819..3746437 Replicon chromosome
Accession NZ_AP022298
Organism Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H7R48_RS17935 Protein ID WP_001291435.1
Coordinates 3746219..3746437 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H7R48_RS17930 Protein ID WP_000344800.1
Coordinates 3745819..3746193 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R48_RS17920 3740908..3742101 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7R48_RS17925 3742124..3745273 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
H7R48_RS17930 3745819..3746193 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H7R48_RS17935 3746219..3746437 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H7R48_RS17940 3746609..3747160 + 552 WP_000102564.1 maltose O-acetyltransferase -
H7R48_RS17945 3747276..3747746 + 471 WP_000136192.1 YlaC family protein -
H7R48_RS17950 3747910..3749460 + 1551 WP_077557245.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H7R48_RS17955 3749502..3749855 - 354 WP_053891426.1 DUF1428 family protein -
H7R48_RS17965 3750234..3750545 + 312 WP_000409911.1 MGMT family protein -
H7R48_RS17970 3750576..3751148 - 573 WP_000779833.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35272 WP_001291435.1 NZ_AP022298:3746219-3746437 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T35272 NZ_AP022298:3746219-3746437 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT35272 WP_000344800.1 NZ_AP022298:3745819-3746193 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT35272 NZ_AP022298:3745819-3746193 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References