Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2401451..2402089 Replicon chromosome
Accession NZ_AP022298
Organism Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)


Gene name hicA Uniprot ID D3GRG9
Locus tag H7R48_RS11445 Protein ID WP_001314712.1
Coordinates 2401451..2401627 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H7R48_RS11450 Protein ID WP_077248684.1
Coordinates 2401673..2402089 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R48_RS11425 2397073..2398284 - 1212 WP_071593600.1 BenE family transporter YdcO -
H7R48_RS11430 2398337..2398873 + 537 WP_000429149.1 DNA-binding transcriptional regulator SutR -
H7R48_RS11435 2398946..2400907 + 1962 WP_001314711.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H7R48_RS11440 2400999..2401229 - 231 WP_000494241.1 YncJ family protein -
H7R48_RS11445 2401451..2401627 + 177 WP_001314712.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H7R48_RS11450 2401673..2402089 + 417 WP_077248684.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H7R48_RS11455 2402168..2403574 + 1407 WP_000760623.1 PLP-dependent aminotransferase family protein -
H7R48_RS11460 2403819..2404964 + 1146 WP_000047443.1 ABC transporter substrate-binding protein -
H7R48_RS11465 2404982..2405995 + 1014 WP_000220407.1 ABC transporter ATP-binding protein -
H7R48_RS11470 2405996..2406937 + 942 WP_001251335.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(87-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6746.81 Da        Isoelectric Point: 11.8888

>T35258 WP_001314712.1 NZ_AP022298:2401451-2401627 [Escherichia coli]
VKQSEFRRWLESQGVNVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T35258 NZ_AP022298:2401451-2401627 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCAATGTAGCAAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTACGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15232.60 Da        Isoelectric Point: 4.7286

>AT35258 WP_077248684.1 NZ_AP022298:2401673-2402089 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSDITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT35258 NZ_AP022298:2401673-2402089 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGACATCACTCAGCAAGAATTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GRG9

Antitoxin

Source ID Structure

References