Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 29568..30127 Replicon plasmid pBEC1-S17-ESBL-07_1
Accession NZ_AP022296
Organism Escherichia coli strain BEC1-S17-ESBL-07

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag H7R29_RS22395 Protein ID WP_000421257.1
Coordinates 29568..29843 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID U9Y625
Locus tag H7R29_RS22400 Protein ID WP_001178087.1
Coordinates 29843..30127 (-) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R29_RS22365 25640..25894 + 255 WP_000343106.1 hypothetical protein -
H7R29_RS22370 25894..26484 + 591 WP_001401589.1 hypothetical protein -
H7R29_RS22375 26697..27749 + 1053 WP_000655519.1 hypothetical protein -
H7R29_RS22380 27925..28527 + 603 WP_000521606.1 hypothetical protein -
H7R29_RS22385 28665..28931 + 267 WP_000627831.1 hypothetical protein -
H7R29_RS22390 29122..29403 - 282 WP_001105066.1 hypothetical protein -
H7R29_RS22395 29568..29843 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R29_RS22400 29843..30127 - 285 WP_001178087.1 ribbon-helix-helix domain-containing protein Antitoxin
H7R29_RS22405 31032..31904 - 873 WP_000729438.1 incFII family plasmid replication initiator RepA -
H7R29_RS22410 32607..33248 + 642 WP_021515453.1 hypothetical protein -
H7R29_RS22415 33388..34050 + 663 WP_005082527.1 hypothetical protein -
H7R29_RS22420 34317..34460 + 144 WP_000176500.1 hypothetical protein -
H7R29_RS22425 34541..34783 + 243 WP_021515452.1 PilI type IV pilus biogenesis protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / blaCTX-M-14 - 1..105884 105884


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T35253 WP_000421257.1 NZ_AP022296:c29843-29568 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T35253 NZ_AP022296:c29843-29568 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10488.92 Da        Isoelectric Point: 5.6558

>AT35253 WP_001178087.1 NZ_AP022296:c30127-29843 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT35253 NZ_AP022296:c30127-29843 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCTCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1PMX5


Antitoxin

Source ID Structure
AlphaFold DB U9Y625

References