Detailed information of TA system TA035248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3462144..3462981
• Replicon: chromosome
• Accession: NZ_AP022295
• Organism: Escherichia coli strain BEC1-S17-ESBL-07

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: H7R29_RS16635
• Protein ID: WP_000227784.1
• Coordinates: 3462439..3462981 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A3T7ZCX8
• Locus tag: H7R29_RS16630
• Protein ID: WP_001442339.1
• Coordinates: 3462144..3462455 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R29_RS16605	3457164..3458111	+	948	WP_001239444.1	cytochrome o ubiquinol oxidase subunit II	-
H7R29_RS16610	3458133..3460124	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
H7R29_RS16615	3460114..3460728	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
H7R29_RS16620	3460728..3461057	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
H7R29_RS16625	3461069..3461959	+	891	WP_000971336.1	heme o synthase	-
H7R29_RS16630	3462144..3462455	+	312	WP_001442339.1	DUF1778 domain-containing protein	Antitoxin
H7R29_RS16635	3462439..3462981	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
H7R29_RS16640	3463037..3463972	-	936	WP_001297127.1	sel1 repeat family protein	-
H7R29_RS16645	3464380..3465744	+	1365	WP_001000983.1	MFS transporter	-
H7R29_RS16650	3465872..3466363	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
H7R29_RS16655	3466531..3467442	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T35248 WP_000227784.1 NZ_AP022295:3462439-3462981 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T35248 NZ_AP022295:3462439-3462981 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT35248 WP_001442339.1 NZ_AP022295:3462144-3462455 [Escherichia coli]
MPGKTATLPDVDKTLKNARVEIKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT35248 NZ_AP022295:3462144-3462455 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAAATCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A3T7ZCX8