Detailed information of TA system TA035236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1309584..1310209
• Replicon: chromosome
• Accession: NZ_AP022295
• Organism: Escherichia coli strain BEC1-S17-ESBL-07

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8S7SZU1
• Locus tag: H7R29_RS06260
• Protein ID: WP_000911327.1
• Coordinates: 1309811..1310209 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: H7R29_RS06255
• Protein ID: WP_000450524.1
• Coordinates: 1309584..1309811 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R29_RS06230	1305386..1305856	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
H7R29_RS06235	1305856..1306428	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
H7R29_RS06240	1306574..1307452	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
H7R29_RS06245	1307469..1308503	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
H7R29_RS06250	1308716..1309429	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
H7R29_RS06255	1309584..1309811	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H7R29_RS06260	1309811..1310209	+	399	WP_000911327.1	tRNA(fMet)-specific endonuclease VapC	Toxin
H7R29_RS06265	1310356..1311219	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
H7R29_RS06270	1311234..1313249	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
H7R29_RS06275	1313323..1314021	+	699	WP_000679823.1	esterase	-
H7R29_RS06280	1314131..1314331	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T35236 WP_000911327.1 NZ_AP022295:1309811-1310209 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMEIIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T35236 NZ_AP022295:1309811-1310209 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGATTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT35236 WP_000450524.1 NZ_AP022295:1309584-1309811 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT35236 NZ_AP022295:1309584-1309811 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33