Detailed information of TA system TA035216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4504703..4505523
• Replicon: chromosome
• Accession: NZ_AP022287
• Organism: Escherichia coli strain WP9-S17-ESBL-11

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: A0A0V9MKL8
• Locus tag: H7R47_RS21610
• Protein ID: WP_001550554.1
• Coordinates: 4504703..4504960 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: H7R47_RS21615
• Protein ID: WP_001519303.1
• Coordinates: 4504972..4505523 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R47_RS21590	4499989..4501095	+	1107	WP_001519308.1	N-acetylneuraminate epimerase	-
H7R47_RS21595	4501160..4502140	+	981	WP_001519307.1	transposase	-
H7R47_RS21600	4502723..4503964	-	1242	Protein_4235	DNA helicase	-
H7R47_RS21605	4504027..4504326	+	300	WP_001519304.1	GNAT family N-acetyltransferase	-
H7R47_RS21610	4504703..4504960	+	258	WP_001550554.1	hypothetical protein	Toxin
H7R47_RS21615	4504972..4505523	+	552	WP_001519303.1	N-acetyltransferase	Antitoxin
H7R47_RS21620	4505575..4505736	+	162	WP_001519302.1	hypothetical protein	-
H7R47_RS21625	4505773..4506321	+	549	WP_001519301.1	hypothetical protein	-
H7R47_RS21630	4506448..4506708	+	261	WP_000084876.1	hypothetical protein	-
H7R47_RS26240	4506746..4506862	+	117	Protein_4242	VOC family protein	-
H7R47_RS21635	4507107..4508228	+	1122	WP_000010833.1	M42 family metallopeptidase	-
H7R47_RS21640	4508225..4508503	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
H7R47_RS21645	4508515..4509828	+	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	4496113..4505736	9623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9744.33 Da
• Isoelectric Point: 11.0090

>T35216 WP_001550554.1 NZ_AP022287:4504703-4504960 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKPVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T35216 NZ_AP022287:4504703-4504960 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGCCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT35216 WP_001519303.1 NZ_AP022287:4504972-4505523 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT35216 NZ_AP022287:4504972-4505523 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A0V9MKL8