Detailed information of TA system TA035215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4461028..4461536
• Replicon: chromosome
• Accession: NZ_AP022287
• Organism: Escherichia coli strain WP9-S17-ESBL-11

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: H7R47_RS21405
• Protein ID: WP_001534843.1
• Coordinates: 4461327..4461536 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: H7R47_RS21400
• Protein ID: WP_000063161.1
• Coordinates: 4461028..4461330 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R47_RS21385	4457556..4459706	+	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-
H7R47_RS21390	4459824..4460027	+	204	WP_001534846.1	YbdD/YjiX family protein	-
H7R47_RS21395	4460038..4460994	+	957	WP_001534845.1	GTPase	-
H7R47_RS21400	4461028..4461330	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
H7R47_RS21405	4461327..4461536	-	210	WP_001534843.1	BrnT family toxin	Toxin
H7R47_RS21410	4461632..4462798	+	1167	WP_001534841.1	restriction endonuclease	-
H7R47_RS21415	4462862..4464481	+	1620	WP_001550561.1	type I restriction-modification system subunit M	-
H7R47_RS21420	4464471..4465781	+	1311	WP_001534837.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T35215 WP_001534843.1 NZ_AP022287:c4461536-4461327 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T35215 NZ_AP022287:c4461536-4461327 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT35215 WP_000063161.1 NZ_AP022287:c4461330-4461028 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT35215 NZ_AP022287:c4461330-4461028 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3