Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3852550..3853168 Replicon chromosome
Accession NZ_AP022287
Organism Escherichia coli strain WP9-S17-ESBL-11

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H7R47_RS18455 Protein ID WP_001291435.1
Coordinates 3852950..3853168 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H7R47_RS18450 Protein ID WP_000344800.1
Coordinates 3852550..3852924 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R47_RS18440 3847655..3850804 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
H7R47_RS18445 3851151..3852131 - 981 WP_000019441.1 IS5-like element ISKpn26 family transposase -
H7R47_RS18450 3852550..3852924 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H7R47_RS18455 3852950..3853168 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H7R47_RS18460 3853340..3853891 + 552 WP_060579167.1 maltose O-acetyltransferase -
H7R47_RS18465 3854007..3854477 + 471 WP_000136192.1 YlaC family protein -
H7R47_RS18470 3854641..3856191 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H7R47_RS18475 3856233..3856586 - 354 WP_001550741.1 DUF1428 family protein -
H7R47_RS18485 3856965..3857276 + 312 WP_000409911.1 MGMT family protein -
H7R47_RS18490 3857307..3857879 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3851151..3852131 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35212 WP_001291435.1 NZ_AP022287:3852950-3853168 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T35212 NZ_AP022287:3852950-3853168 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT35212 WP_000344800.1 NZ_AP022287:3852550-3852924 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT35212 NZ_AP022287:3852550-3852924 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References