Detailed information of TA system TA035189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 1178..1871
• Replicon: plasmid pWP8-W19-CRE-03_3
• Accession: NZ_AP022284
• Organism: Aeromonas veronii strain WP8-W19-CRE-03

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: H7R74_RS22110
• Protein ID: WP_000182276.1
• Coordinates: 1515..1871 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: H7R74_RS22105
• Protein ID: WP_001172026.1
• Coordinates: 1178..1513 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R74_RS22090	101..481	-	381	WP_001054412.1	hypothetical protein	-
H7R74_RS22095	478..804	-	327	WP_000091614.1	hypothetical protein	-
H7R74_RS22100	828..1163	-	336	WP_000741275.1	hypothetical protein	-
H7R74_RS22105	1178..1513	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
H7R74_RS22110	1515..1871	-	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R74_RS22115	2063..2665	+	603	WP_010465829.1	recombinase family protein	-
H7R74_RS22120	2649..5678	+	3030	WP_032432545.1	Tn3 family transposase	-
H7R74_RS22125	5635..6531	-	897	WP_182963889.1	relaxase/mobilization nuclease domain-containing protein	-
H7R74_RS22130	6512..6850	-	339	WP_128318466.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12173	12173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T35189 WP_000182276.1 NZ_AP022284:c1871-1515 [Aeromonas veronii]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T35189 NZ_AP022284:c1871-1515 [Aeromonas veronii]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT35189 WP_001172026.1 NZ_AP022284:c1513-1178 [Aeromonas veronii]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT35189 NZ_AP022284:c1513-1178 [Aeromonas veronii]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7