Detailed information of TA system TA035187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 17339..18032
• Replicon: plasmid pWP8-W19-CRE-02_3
• Accession: NZ_AP022277
• Organism: Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: H7R70_RS25035
• Protein ID: WP_000182276.1
• Coordinates: 17339..17695 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: H7R70_RS25040
• Protein ID: WP_001172026.1
• Coordinates: 17697..18032 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R70_RS25025	13532..16561	-	3030	WP_032432545.1	Tn3 family transposase	-
H7R70_RS25030	16545..17147	-	603	WP_010465829.1	recombinase family protein	-
H7R70_RS25035	17339..17695	+	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R70_RS25040	17697..18032	+	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
H7R70_RS25045	18047..18382	+	336	WP_000741275.1	hypothetical protein	-
H7R70_RS25050	18406..18732	+	327	WP_000091614.1	hypothetical protein	-
H7R70_RS25055	18729..19109	+	381	WP_001054412.1	hypothetical protein	-
H7R70_RS25060	19227..19508	+	282	WP_182947067.1	DUF3391 domain-containing protein	-
H7R70_RS25065	19547..20461	+	915	WP_182947068.1	HD domain-containing protein	-
H7R70_RS25070	20629..20949	-	321	Protein_26	DUF4158 domain-containing protein	-
H7R70_RS25075	20953..21363	-	411	WP_003150546.1	putative toxin-antitoxin system toxin component, PIN family	-
H7R70_RS25080	21363..21602	-	240	WP_003089107.1	ribbon-helix-helix domain-containing protein	-
H7R70_RS25085	21761..22012	+	252	WP_130627584.1	type II toxin-antitoxin system YafQ family toxin	-
H7R70_RS25090	22139..22714	+	576	WP_001173919.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2	-	1..46974	46974
	-	inside	IScluster/Tn	-	-	10822..23869	13047

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T35187 WP_000182276.1 NZ_AP022277:17339-17695 [Enterobacter cloacae]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T35187 NZ_AP022277:17339-17695 [Enterobacter cloacae]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT35187 WP_001172026.1 NZ_AP022277:17697-18032 [Enterobacter cloacae]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT35187 NZ_AP022277:17697-18032 [Enterobacter cloacae]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7