Detailed information of TA system TA035186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 118796..119535
• Replicon: plasmid pWP8-W19-CRE-02_2
• Accession: NZ_AP022276
• Organism: Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5E1AVR5
• Locus tag: H7R70_RS24895
• Protein ID: WP_013087172.1
• Coordinates: 119050..119535 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: H7R70_RS24890
• Protein ID: WP_012540086.1
• Coordinates: 118796..119062 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R70_RS24860	114027..114563	-	537	WP_182947060.1	GNAT family N-acetyltransferase	-
H7R70_RS24865	114688..116439	-	1752	WP_162875198.1	arsenical pump-driving ATPase	-
H7R70_RS24870	116466..116828	-	363	WP_013087168.1	arsenic metallochaperone ArsD family protein	-
H7R70_RS24875	116904..117449	-	546	WP_013087169.1	sigma-70 family RNA polymerase sigma factor	-
H7R70_RS24880	117458..118171	-	714	WP_182947061.1	arsenical resistance protein ArsH	-
H7R70_RS24885	118173..118496	-	324	WP_013087171.1	metalloregulator ArsR/SmtB family transcription factor	-
H7R70_RS24890	118796..119062	+	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
H7R70_RS24895	119050..119535	+	486	WP_013087172.1	GNAT family N-acetyltransferase	Toxin
H7R70_RS24900	119954..121294	+	1341	WP_000589001.1	ISNCY family transposase	-
H7R70_RS24905	121570..122397	-	828	WP_047365775.1	universal stress protein	-
H7R70_RS24910	122416..123894	-	1479	WP_047365777.1	SulP family inorganic anion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib	-	1..128763	128763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17810.62 Da
• Isoelectric Point: 9.8560

>T35186 WP_013087172.1 NZ_AP022276:119050-119535 [Enterobacter cloacae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T35186 NZ_AP022276:119050-119535 [Enterobacter cloacae]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT35186 WP_012540086.1 NZ_AP022276:118796-119062 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT35186 NZ_AP022276:118796-119062 [Enterobacter cloacae]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A5E1AVR5

Antitoxin

• AlphaFold DB: A0A5E1AWX8