Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 118796..119535 Replicon plasmid pWP8-W19-CRE-02_2
Accession NZ_AP022276
Organism Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag H7R70_RS24895 Protein ID WP_013087172.1
Coordinates 119050..119535 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag H7R70_RS24890 Protein ID WP_012540086.1
Coordinates 118796..119062 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R70_RS24860 114027..114563 - 537 WP_182947060.1 GNAT family N-acetyltransferase -
H7R70_RS24865 114688..116439 - 1752 WP_162875198.1 arsenical pump-driving ATPase -
H7R70_RS24870 116466..116828 - 363 WP_013087168.1 arsenic metallochaperone ArsD family protein -
H7R70_RS24875 116904..117449 - 546 WP_013087169.1 sigma-70 family RNA polymerase sigma factor -
H7R70_RS24880 117458..118171 - 714 WP_182947061.1 arsenical resistance protein ArsH -
H7R70_RS24885 118173..118496 - 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
H7R70_RS24890 118796..119062 + 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
H7R70_RS24895 119050..119535 + 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
H7R70_RS24900 119954..121294 + 1341 WP_000589001.1 ISNCY family transposase -
H7R70_RS24905 121570..122397 - 828 WP_047365775.1 universal stress protein -
H7R70_RS24910 122416..123894 - 1479 WP_047365777.1 SulP family inorganic anion transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib - 1..128763 128763


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T35186 WP_013087172.1 NZ_AP022276:119050-119535 [Enterobacter cloacae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T35186 NZ_AP022276:119050-119535 [Enterobacter cloacae]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT35186 WP_012540086.1 NZ_AP022276:118796-119062 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT35186 NZ_AP022276:118796-119062 [Enterobacter cloacae]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References