Detailed information of TA system TA035185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 86229..86866
• Replicon: plasmid pWP8-W19-CRE-02_2
• Accession: NZ_AP022276
• Organism: Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A9E6WXZ4
• Locus tag: H7R70_RS24685
• Protein ID: WP_032662169.1
• Coordinates: 86229..86639 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A9E6WTF5
• Locus tag: H7R70_RS24690
• Protein ID: WP_032662167.1
• Coordinates: 86636..86866 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R70_RS24640	82069..82398	-	330	Protein_87	IS3 family transposase	-
H7R70_RS24645	82398..82586	+	189	Protein_88	Tn3 family transposase	-
H7R70_RS24650	82623..82796	+	174	WP_137493210.1	hypothetical protein	-
H7R70_RS24655	82825..83322	+	498	WP_126547899.1	disulfide bond formation protein B	-
H7R70_RS24660	83386..83784	-	399	WP_126547900.1	GNAT family N-acetyltransferase	-
H7R70_RS24665	83825..84226	-	402	Protein_92	phosphotransferase	-
H7R70_RS24670	84333..84662	-	330	Protein_93	IS3 family transposase	-
H7R70_RS24675	84662..84850	+	189	Protein_94	Tn3 family transposase	-
H7R70_RS24680	84884..86155	-	1272	Protein_95	ATP-dependent endonuclease	-
H7R70_RS24685	86229..86639	-	411	WP_032662169.1	PIN domain-containing protein	Toxin
H7R70_RS24690	86636..86866	-	231	WP_032662167.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H7R70_RS24695	87408..87698	+	291	WP_013087126.1	hypothetical protein	-
H7R70_RS24700	87829..88359	+	531	WP_013087127.1	hypothetical protein	-
H7R70_RS24705	88378..89154	+	777	WP_015572054.1	site-specific integrase	-
H7R70_RS24710	89356..89613	+	258	Protein_101	site-specific integrase	-
H7R70_RS24715	89857..90867	-	1011	WP_015572055.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib	-	1..128763	128763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14729.03 Da
• Isoelectric Point: 5.7351

>T35185 WP_032662169.1 NZ_AP022276:c86639-86229 [Enterobacter cloacae]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 411 bp

>T35185 NZ_AP022276:c86639-86229 [Enterobacter cloacae]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTGGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8645.77 Da
• Isoelectric Point: 4.4692

>AT35185 WP_032662167.1 NZ_AP022276:c86866-86636 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT35185 NZ_AP022276:c86866-86636 [Enterobacter cloacae]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA