Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 48509..49248 Replicon plasmid pWP8-W19-CRE-02_2
Accession NZ_AP022276
Organism Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)


Gene name tacT Uniprot ID A0A411GR51
Locus tag H7R70_RS24470 Protein ID WP_032664832.1
Coordinates 48509..48994 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A411GR70
Locus tag H7R70_RS24475 Protein ID WP_032664831.1
Coordinates 48982..49248 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R70_RS24455 44596..45984 + 1389 WP_182947064.1 AAA family ATPase -
H7R70_RS24460 46489..46899 + 411 WP_182947065.1 hypothetical protein -
H7R70_RS24465 46955..48301 - 1347 WP_182947066.1 ISNCY family transposase -
H7R70_RS24470 48509..48994 - 486 WP_032664832.1 GNAT family N-acetyltransferase Toxin
H7R70_RS24475 48982..49248 - 267 WP_032664831.1 DUF1778 domain-containing protein Antitoxin
H7R70_RS24480 49903..50916 + 1014 WP_032664830.1 replication initiation protein -
H7R70_RS24485 51809..52612 + 804 WP_032664829.1 DUF4225 domain-containing protein -
H7R70_RS24490 52590..52913 - 324 WP_032664828.1 hypothetical protein -
H7R70_RS24495 52928..53698 - 771 WP_032664827.1 conjugal transfer protein TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib - 1..128763 128763
- flank IS/Tn - - 46955..48313 1358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-147)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17593.31 Da        Isoelectric Point: 9.6084

>T35184 WP_032664832.1 NZ_AP022276:c48994-48509 [Enterobacter cloacae]
VGRVTAPEPLSSCHQLAEFASGETVLDDWLKQRGLKNQALGAARTFVVCKANTKQVAGFYSLATGSVNHVEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKSFYLHHGFKPSQTQERTLFLKLP
Q

Download         Length: 486 bp

>T35184 NZ_AP022276:c48994-48509 [Enterobacter cloacae]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGTTGCCATCAACTTGCTGAGTTCGCCAGTGGTGAGACTGTACTTGA
TGACTGGCTTAAACAGAGAGGATTGAAAAACCAGGCTCTGGGGGCTGCCCGAACGTTCGTAGTTTGTAAAGCAAACACGA
AACAGGTTGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAACGGGAAGTCTCAGGCGCAACATG
CCAGATCCTATTCCGGTAATCATACTTGCTCGCCTGGCAGTCGATGTTTCATTCCGGGGAAAGGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTATCGTGTAGCTGAGAATATTGGAGTCCGTGCAATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAGTTTTTATCTTCATCATGGCTTTAAGCCATCGCAAACCCAGGAACGTACACTGTTTCTCAAGTTACCA
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10077.50 Da        Isoelectric Point: 4.4733

>AT35184 WP_032664831.1 NZ_AP022276:c49248-48982 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADNVAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT35184 NZ_AP022276:c49248-48982 [Enterobacter cloacae]
ATGAAATCAGATGTTCAGCTCAACCTCAGAGCCAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACAGACTTCATTCTGGAAATGGCATGCCAGGCGGCCGAGAATGTTATCCTCGACCGTCGCGTTTTTAATT
TTAACGATGAGCAGTACGCAGAGTTTATTGATATGCTCGATGCACCAGTTGCGGATAACGTTGCCATTGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A411GR51

Antitoxin

Source ID Structure
AlphaFold DB A0A411GR70

References