Detailed information of TA system TA035183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 110402..111141
• Replicon: plasmid pWP8-W19-CRE-02_1
• Accession: NZ_AP022275
• Organism: Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: H7R70_RS24105
• Protein ID: WP_182947004.1
• Coordinates: 110402..110887 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: W7NLL2
• Locus tag: H7R70_RS24110
• Protein ID: WP_014172304.1
• Coordinates: 110875..111141 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R70_RS24080	105486..106127	-	642	WP_045295096.1	TerD family protein	-
H7R70_RS24085	106150..106788	-	639	WP_045295093.1	VWA domain-containing protein	-
H7R70_RS24090	107113..108000	+	888	WP_131638989.1	hypothetical protein	-
H7R70_RS24095	108399..108809	+	411	WP_063402002.1	hypothetical protein	-
H7R70_RS24100	108864..110152	-	1289	Protein_110	ISNCY family transposase	-
H7R70_RS24105	110402..110887	-	486	WP_182947004.1	GNAT family N-acetyltransferase	Toxin
H7R70_RS24110	110875..111141	-	267	WP_014172304.1	DUF1778 domain-containing protein	Antitoxin
H7R70_RS24115	111384..111623	-	240	WP_182947005.1	type II toxin-antitoxin system antitoxin RelB	-
H7R70_RS24120	112001..112405	-	405	WP_182947058.1	H-NS histone family protein	-
H7R70_RS24125	112978..113205	-	228	WP_182947006.1	hypothetical protein	-
H7R70_RS24130	113506..113724	-	219	WP_012543040.1	cold-shock protein	-
H7R70_RS24135	114003..114215	+	213	WP_001446195.1	cold shock-like protein CspF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..132349	132349
	-	flank	IS/Tn	-	-	108864..109703	839

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (80-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17505.23 Da
• Isoelectric Point: 10.1390

>T35183 WP_182947004.1 NZ_AP022275:c110887-110402 [Enterobacter cloacae]
VGRVTVPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHVEATGSLRRNM
PEPIPVIILARLAVDASFRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEKAKGFYLHHGFKASQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T35183 NZ_AP022275:c110887-110402 [Enterobacter cloacae]
GTGGGACGTGTAACAGTACCGGAACCTTTATCCAGCTCACATCAACTGGCTGAGTTCGTCAGCGGGGAGGCCGTTCTCGA
TGACTGGCTTAAACAGCGAGGGTTAAAAAATCAGGCACTTGGTGCTGCCAGAACATTCGTTGTATGTAAAACAGGCACGA
AACAGGTTGCCGGTTTTTACTCCCTGGCCACCGGCAGCGTTAACCATGTAGAAGCGACGGGAAGTCTCAGGCGCAATATG
CCAGAACCAATCCCGGTAATCATACTTGCTCGACTGGCTGTCGATGCCTCATTTCGTGGACAAGGGCTGGGGGCTGATTT
ACTGCACGATGCAGTATTGCGCTGTTATCGTGTGGCTGAAAATATAGGCGTTCGTGCAATTATGGTTCATGCGCTGACAG
AAAAAGCCAAAGGGTTTTATCTTCATCATGGTTTTAAGGCATCGCAAACCCAGGAACGGACTCTGTTTCTCAAGCTTCCG
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10049.45 Da
• Isoelectric Point: 4.4733

>AT35183 WP_014172304.1 NZ_AP022275:c111141-110875 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDAAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT35183 NZ_AP022275:c111141-110875 [Enterobacter cloacae]
ATGAAATCAGATGTTCAGCTCAACCTCAGAGCCAAGGAGTCTCAAAGAGCACTTATCGATGCTGCTGCAGAAATCCTTCA
TAAATCACGTACAGACTTTATCCTTGAAATGGCATGCCAGGCTGCTGAGAATGTGATCCTCGATCGTCGCGTATTCAATT
TTAACGATGAGCAGTACGCTGAGTTTATCGATATGCTCGATGCGCCAGTTGCGGATGATGCTGCCATCAATAAACTCCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: W7NLL2