Detailed information of TA system TA035182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 17485..18289
• Replicon: plasmid pWP8-W19-CRE-02_1
• Accession: NZ_AP022275
• Organism: Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: H7R70_RS23645
• Protein ID: WP_182947026.1
• Coordinates: 17759..18289 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: H7R70_RS23640
• Protein ID: WP_182947025.1
• Coordinates: 17485..17754 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R70_RS23615	13351..13599	+	249	WP_182947021.1	hypothetical protein	-
H7R70_RS23620	14041..14244	+	204	WP_182947022.1	host cell division inhibitor Icd-like protein	-
H7R70_RS23625	14241..14474	+	234	WP_182947023.1	hypothetical protein	-
H7R70_RS23630	14537..15331	+	795	WP_182947055.1	Replication protein repL	-
H7R70_RS23635	15990..16907	+	918	WP_182947024.1	RepB family plasmid replication initiator protein	-
H7R70_RS23640	17485..17754	+	270	WP_182947025.1	DUF1778 domain-containing protein	Antitoxin
H7R70_RS23645	17759..18289	+	531	WP_182947026.1	GNAT family N-acetyltransferase	Toxin
H7R70_RS23650	18535..18738	-	204	WP_049002001.1	hypothetical protein	-
H7R70_RS23655	18750..19373	-	624	WP_032639997.1	ParA family protein	-
H7R70_RS23660	19583..19969	-	387	WP_151413794.1	hypothetical protein	-
H7R70_RS23665	20014..20973	-	960	WP_182947056.1	recombinase	-
H7R70_RS23670	22639..22941	-	303	WP_047626395.1	HigA family addiction module antidote protein	-
H7R70_RS23675	22941..23222	-	282	WP_089601432.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..132349	132349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-157)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20157.93 Da
• Isoelectric Point: 6.3396

>T35182 WP_182947026.1 NZ_AP022275:17759-18289 [Enterobacter cloacae]
VEGLKVEIFSEEVEYDVSNFDCGEEYLNTFLTDHLKRQHSGKFLLGYVLVTREKKPRVVGYYTLSGSCFEKEYFPSKTQQ
KRVPYKNVPSVTLGRLAIDNSIQRQGYGETLVTHAMKVVYHASQAVGIHGMFVEALSDDAKKFYLGLGFILLKREYSNAL
FYPTKSIEELFEAQDE

Nucleotide

• Length: 531 bp

>T35182 NZ_AP022275:17759-18289 [Enterobacter cloacae]
GTGGAAGGTTTAAAGGTAGAAATTTTTTCTGAGGAAGTTGAGTATGATGTAAGCAACTTCGATTGCGGTGAAGAGTATCT
TAATACTTTTCTCACAGACCATCTGAAACGCCAGCACAGTGGCAAGTTTTTGCTTGGTTATGTGCTCGTCACGAGAGAGA
AAAAACCTCGGGTTGTGGGGTACTACACATTGTCTGGGAGCTGCTTCGAAAAGGAGTATTTCCCGAGCAAGACTCAGCAA
AAACGTGTGCCCTATAAAAACGTTCCCAGTGTAACGCTGGGGCGTTTGGCAATTGACAATAGCATTCAACGTCAAGGGTA
TGGAGAAACGCTTGTAACACATGCCATGAAGGTTGTGTATCACGCATCCCAGGCAGTAGGAATACATGGAATGTTTGTTG
AAGCTCTCAGCGACGACGCCAAAAAATTCTACCTTGGCCTTGGCTTCATCCTGCTCAAGCGCGAATATAGTAACGCCCTC
TTCTATCCAACCAAATCGATAGAAGAATTATTCGAAGCTCAAGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10136.50 Da
• Isoelectric Point: 7.0158

>AT35182 WP_182947025.1 NZ_AP022275:17485-17754 [Enterobacter cloacae]
MSALKKQRIDLRLNEDDKSMIEEAAAMTNQSISQFMVSTASERAAEIINQHRRQILNEESWNAVMEAISNPPAPNDRLKK
AAKRLQNLE

Nucleotide

• Length: 270 bp

>AT35182 NZ_AP022275:17485-17754 [Enterobacter cloacae]
ATGTCCGCACTGAAGAAACAGCGCATCGATCTTAGATTAAATGAAGACGACAAGAGCATGATCGAAGAAGCCGCGGCAAT
GACTAACCAGTCAATTTCACAGTTCATGGTTAGCACTGCCTCTGAACGTGCAGCGGAAATTATTAACCAGCACCGCCGCC
AGATCCTCAACGAGGAGTCATGGAACGCGGTTATGGAAGCGATCAGTAACCCGCCAGCACCGAACGACCGACTGAAAAAA
GCTGCTAAGCGTCTCCAGAATTTGGAGTAA