Detailed information of TA system TA035180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4695631..4696235
• Replicon: chromosome
• Accession: NZ_AP022274
• Organism: Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2T4Y605
• Locus tag: H7R70_RS22655
• Protein ID: WP_041911637.1
• Coordinates: 4696050..4696235 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7R70_RS22650
• Protein ID: WP_108418126.1
• Coordinates: 4695631..4696035 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R70_RS22635	4691171..4691989	-	819	WP_109492507.1	helix-turn-helix domain-containing protein	-
H7R70_RS22640	4692215..4693615	+	1401	WP_182946075.1	MFS transporter	-
H7R70_RS22645	4693626..4695575	+	1950	WP_182946076.1	glycoside hydrolase family 127 protein	-
H7R70_RS22650	4695631..4696035	-	405	WP_108418126.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H7R70_RS22655	4696050..4696235	-	186	WP_041911637.1	type II toxin-antitoxin system HicA family toxin	Toxin
H7R70_RS22660	4696445..4697185	-	741	WP_089599697.1	MipA/OmpV family protein	-
H7R70_RS22665	4697303..4698268	+	966	WP_100165410.1	LysR family transcriptional regulator	-
H7R70_RS22670	4698265..4699803	-	1539	WP_042716601.1	aldehyde dehydrogenase	-
H7R70_RS22675	4699969..4700853	+	885	WP_042718576.1	ROK family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6852.04 Da
• Isoelectric Point: 11.2341

>T35180 WP_041911637.1 NZ_AP022274:c4696235-4696050 [Enterobacter cloacae]
VKSADVITVLIGHGWKCVRTKGSHHQFRHPEQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Nucleotide

• Length: 186 bp

>T35180 NZ_AP022274:c4696235-4696050 [Enterobacter cloacae]
GTGAAAAGTGCGGATGTCATTACTGTTCTGATCGGCCATGGCTGGAAATGTGTCAGAACGAAAGGAAGCCATCACCAGTT
TCGCCATCCCGAACAGAAAGGGCTGGTTACTGTCCCACATCCTAAGAAGGATATTAAACCGGGCACTCTCGCCCAGATCT
GGCGGCAGGCAGGAATTAAACACTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14970.71 Da
• Isoelectric Point: 4.3197

>AT35180 WP_108418126.1 NZ_AP022274:c4696035-4695631 [Enterobacter cloacae]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDDALPLPGNVEAHLESRPEDFTGGQWL
LVDINMKQFDGRAERINITMPRRLLVKIDSYVSEHPQFGNRSAFLAEAARRVLP

Nucleotide

• Length: 405 bp

>AT35180 NZ_AP022274:c4696035-4695631 [Enterobacter cloacae]
ATGTTTTATCCGGCTTACATTCATTCTGACTCCGACGGTAGCGCCAGCGGTTTTTTCCCTGACGTTCCAGGCTGCTTTTT
TGCAGGTGATTCCCTGGACGACGCTTTTCAGGATGCACGTGATGCGCTCACCGCGCACTTCGAAGCGCTGTTTGAAATGG
ATGATGCACTGCCTCTTCCGGGTAATGTGGAAGCGCATCTGGAAAGTCGTCCTGAGGATTTCACCGGCGGGCAATGGCTG
TTGGTGGATATTAATATGAAACAGTTCGACGGCAGGGCCGAGCGTATCAACATCACGATGCCTCGACGTTTGCTGGTAAA
GATTGATTCCTATGTCAGCGAGCATCCTCAGTTCGGTAATCGAAGCGCATTTCTGGCTGAGGCAGCACGTCGCGTGCTGC
CGTAG

Structures

Toxin

• AlphaFold DB: A0A2T4Y605