Detailed information of TA system TA035167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1064383..1065003
• Replicon: chromosome
• Accession: NZ_AP022274
• Organism: Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V3PUI7
• Locus tag: H7R70_RS04990
• Protein ID: WP_010428174.1
• Coordinates: 1064383..1064601 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V3PBI9
• Locus tag: H7R70_RS04995
• Protein ID: WP_008499288.1
• Coordinates: 1064629..1065003 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R70_RS04960	1060393..1060653	+	261	WP_010428154.1	type B 50S ribosomal protein L31	-
H7R70_RS04965	1060656..1060796	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
H7R70_RS04970	1060793..1061503	-	711	WP_182946405.1	GNAT family N-acetyltransferase	-
H7R70_RS04975	1061605..1063065	+	1461	WP_182946406.1	PLP-dependent aminotransferase family protein	-
H7R70_RS04980	1063037..1063504	-	468	WP_089597999.1	hypothetical protein	-
H7R70_RS04985	1063622..1064173	-	552	WP_182946407.1	maltose O-acetyltransferase	-
H7R70_RS04990	1064383..1064601	-	219	WP_010428174.1	hemolysin expression modulator Hha	Toxin
H7R70_RS04995	1064629..1065003	-	375	WP_008499288.1	Hha toxicity modulator TomB	Antitoxin
H7R70_RS05000	1065513..1068659	-	3147	WP_042716155.1	multidrug efflux RND transporter permease subunit	-
H7R70_RS05005	1068682..1069875	-	1194	WP_042716154.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8597.97 Da
• Isoelectric Point: 8.9008

>T35167 WP_010428174.1 NZ_AP022274:c1064601-1064383 [Enterobacter cloacae]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T35167 NZ_AP022274:c1064601-1064383 [Enterobacter cloacae]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCTTGCGACGTTGTCAGTCAATAGACACGCTCGAACGTGT
CATCGAGAAAAATAAATACGAGCTATCTGATAATGAACTGGCTGTATTTTATTCAGCCGCTGACCATCGGCTCGCTGAGC
TGACCATGAATAAGCTGTATGACAAAATCCCAACGTCCGTATGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14481.28 Da
• Isoelectric Point: 4.8886

>AT35167 WP_008499288.1 NZ_AP022274:c1065003-1064629 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT35167 NZ_AP022274:c1065003-1064629 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTGAATGACCCAACGTCTGCGATCAATTTACAGTTGAACGAGCTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGACGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGCTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGCTTCGTCAACGTGAGCAGAGCAAACCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: V3PUI7

Antitoxin

• AlphaFold DB: V3PBI9