Detailed information of TA system TA035166

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 539543..540246
• Replicon: chromosome
• Accession: NZ_AP022274
• Organism: Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A6S5XK15
• Locus tag: H7R70_RS02605
• Protein ID: WP_071998139.1
• Coordinates: 539905..540246 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: H7R70_RS02600
• Protein ID: WP_125369523.1
• Coordinates: 539543..539884 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R70_RS02560	535095..535799	+	705	WP_125369513.1	DeoR family transcriptional regulator	-
H7R70_RS02565	535799..536368	+	570	WP_125369847.1	hypothetical protein	-
H7R70_RS02570	536383..536835	+	453	WP_048236233.1	hypothetical protein	-
H7R70_RS02575	536832..537281	+	450	WP_125369515.1	hypothetical protein	-
H7R70_RS02580	537353..537583	+	231	WP_063925496.1	DUF905 domain-containing protein	-
H7R70_RS02585	537682..538521	+	840	WP_125369517.1	DUF945 domain-containing protein	-
H7R70_RS02590	538534..538977	+	444	WP_182946259.1	antirestriction protein	-
H7R70_RS02595	538990..539532	+	543	WP_125369521.1	DNA repair protein RadC	-
H7R70_RS02600	539543..539884	+	342	WP_125369523.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H7R70_RS02605	539905..540246	+	342	WP_071998139.1	TA system toxin CbtA family protein	Toxin
H7R70_RS02610	540504..541511	+	1008	WP_047041139.1	restriction endonuclease	-
H7R70_RS02615	541641..542603	-	963	WP_182946260.1	tryptophan--tRNA ligase	-
H7R70_RS02620	542803..543228	+	426	WP_182946261.1	FosA/FosA2 family fosfomycin resistance glutathione transferase	-
H7R70_RS02625	543214..544221	-	1008	WP_182946262.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	511992..541511	29519

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12710.63 Da
• Isoelectric Point: 6.4514

>T35166 WP_071998139.1 NZ_AP022274:539905-540246 [Enterobacter cloacae]
MKTLPATTPQAATLCLSPVAVWQMLLACLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 342 bp

>T35166 NZ_AP022274:539905-540246 [Enterobacter cloacae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCCGTAGCAGTGTGGCAAATGCTGCTTGC
ATGCCTGCTGGAACAACATTATGGCCTTACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTAGATATTCTGCGGGCGCGGCAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACAGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12898.38 Da
• Isoelectric Point: 5.8853

>AT35166 WP_125369523.1 NZ_AP022274:539543-539884 [Enterobacter cloacae]
MKSTDSENDSFLKWGLNRNVTPFFGARLVQEGNRLHYLADRASISGEFSNEESLKLDEVFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT35166 NZ_AP022274:539543-539884 [Enterobacter cloacae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGGCTGAATCGTAATGTGACGCCTTTCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTGCACTATCTGGCTGACCGGGCCAGCATTAGCGGTGAATTCAGTAACGAAGAATCTT
TGAAGTTGGATGAAGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCATCGTTAA

Structures

Toxin

• AlphaFold DB: A0A6S5XK15