Detailed information of TA system TA035165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 478572..479088
• Replicon: chromosome
• Accession: NZ_AP022274
• Organism: Enterobacter cloacae strain WP8-W19-CRE-02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H7R70_RS02315
• Protein ID: WP_182946244.1
• Coordinates: 478804..479088 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V3EXX3
• Locus tag: H7R70_RS02310
• Protein ID: WP_008501400.1
• Coordinates: 478572..478814 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R70_RS02295	474572..475312	+	741	WP_100165612.1	KDGP aldolase family protein	-
H7R70_RS02300	475428..476564	+	1137	WP_100165611.1	lactonase family protein	-
H7R70_RS02305	476584..478494	+	1911	WP_042718623.1	BglG family transcription antiterminator	-
H7R70_RS02310	478572..478814	+	243	WP_008501400.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H7R70_RS02315	478804..479088	+	285	WP_182946244.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R70_RS02320	479092..479556	-	465	WP_100165609.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
H7R70_RS02325	479655..481793	-	2139	WP_182946245.1	anaerobic ribonucleoside-triphosphate reductase	-
H7R70_RS02330	482169..483812	-	1644	WP_182946246.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10894.73 Da
• Isoelectric Point: 10.3031

>T35165 WP_182946244.1 NZ_AP022274:478804-479088 [Enterobacter cloacae]
MTYELAFDPRALKEWSKLGETVKDKFKKKLADVLVNPRVESARLHGLPDCYKIKLRSQGYRLVYQVQDNVVTVFVIAIGK
REKSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T35165 NZ_AP022274:478804-479088 [Enterobacter cloacae]
ATGACTTATGAACTGGCGTTTGATCCGAGGGCGTTAAAGGAGTGGAGCAAGCTCGGGGAGACTGTAAAAGACAAGTTTAA
GAAGAAACTTGCGGATGTGTTGGTGAACCCAAGGGTCGAATCGGCCCGCCTGCATGGTCTACCGGATTGCTACAAAATAA
AACTCAGATCGCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAATGTTGTAACGGTATTTGTTATCGCTATCGGTAAG
AGAGAGAAATCAGCCGTTTATCATGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8972.37 Da
• Isoelectric Point: 5.0651

>AT35165 WP_008501400.1 NZ_AP022274:478572-478814 [Enterobacter cloacae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT35165 NZ_AP022274:478572-478814 [Enterobacter cloacae]
ATGGCCACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTACGCCGTGCTGGAAAAACTCAACATCACCCC
TACGGAAGCGGTACGATTGCTGTTTCAGTACGTAGCTGAAACAGGACGCATGCCCGTGAAAACGGTCACGATTAGTGACA
GCGAAGATGCGCTGATACAGACGGTCCGGGAGCGGCTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: V3EXX3